Overview

  • Product name

  • Description

    Rabbit polyclonal to Beclin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Beclin 1 (internal sequence).
    Database link: Q14457

  • Positive control

    • WB: Rat liver lysate; HeLa cell lysate. ICC/IF: SK-N-BE cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Predicted molecular weight: 52 kDa.

Target

  • Function

    Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the beclin family.
  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • APG6 antibody
    • ATG 6 antibody
    • ATG6 antibody
    • ATG6 autophagy related 6 homolog antibody
    • Bcl-2-interacting protein beclin antibody
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
    • Beclin 1 autophagy related antibody
    • Beclin-1 antibody
    • Beclin1 antibody
    • BECN 1 antibody
    • Becn1 antibody
    • BECN1_HUMAN antibody
    • Coiled coil myosin like BCL2 interacting protein antibody
    • Coiled-coil myosin-like BCL2-interacting protein antibody
    • GT197 antibody
    • Protein GT197 antibody
    • VPS 30 antibody
    • VPS30 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for Beclin 1 (green) using ab223348 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223348 (D) Merge.

  • Anti-Beclin 1 antibody (ab223348) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit: HRP at 1/2000 dilution

    Predicted band size: 52 kDa



    Blocking: 5% milk + TBST for 1 hour at RT.

    Color Development: TMB solution for 12 minutes at RT.

  • Anti-Beclin 1 antibody (ab223348) at 1/1000 dilution + Rat liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit: HRP at 1/2000 dilution

    Predicted band size: 52 kDa



    Blocking: 5% milk + TBST for 1 hour at RT.

    Color Development: TMB solution for 12 minutes at RT.

References

ab223348 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223348.
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