Anti-Beclin 1 antibody (ab223372)
Key features and details
- Rabbit polyclonal to Beclin 1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Beclin 1 antibody
See all Beclin 1 primary antibodies -
Description
Rabbit polyclonal to Beclin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Beclin 1 (C terminal). From the C-terminus.
Database link: Q14457 -
Positive control
- WB: HEK-293T and HeLa cell lysates. ICC/IF: SK-N-BE cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 52 kDa).
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ICC/IF |
1/100.
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Notes |
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WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 52 kDa). |
ICC/IF
1/100. |
Target
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Function
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the beclin family. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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Database links
- Entrez Gene: 8678 Human
- Omim: 604378 Human
- SwissProt: Q14457 Human
- Unigene: 716464 Human
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Alternative names
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
Images
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4% formaldehyde-fixed SK-N-BE cells stained for Beclin 1 (green) using ab223372 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain (red) at 1/1000 for 60 minutes at room temperature; DAPI (blue) nuclear stain at 1/5000 for 5 minutes at room temperature.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223372 (D) Merge.
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Anti-Beclin 1 antibody (ab223372) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesBlocking buffer: 5% milk in TBST.
Color Development: TMB solution.
Primary and secondary antibodies were incubated for 1 hour at room temperature.
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Anti-Beclin 1 antibody (ab223372) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesBlocking buffer: 5% milk in TBST.
Color Development: TMB solution.
Primary and secondary antibodies were incubated for 1 hour at room temperature.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab223372 has been referenced in 1 publication.
- Yang L et al. Exercise Ameliorates Insulin Resistance of Type 2 Diabetes through Motivating Short-Chain Fatty Acid-Mediated Skeletal Muscle Cell Autophagy. Biology (Basel) 9:N/A (2020). PubMed: 32756447