Key features and details
- Rabbit polyclonal to Beclin 1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Beclin 1 antibody
See all Beclin 1 primary antibodies
DescriptionRabbit polyclonal to Beclin 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat, Chicken, Cow, Orangutan
Recombinant fragment (His-T7-tag) corresponding to Human Beclin 1 aa 1-273. Expressed in E.coli. N-terminal tags.
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-MEGSKTSNNSTMQ VSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETN SGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLS RRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYK RCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLE KVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDK LKKTNVFNAT
Database link: Q14457
- WB: A431, HeLa and MCF7 cell lysate; Porcine brain tissue. Recombinant human Beclin 1 protein. IHC-P: Human cerebrum, stomach and pancreas tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesFollowed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
FunctionPlays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Sequence similaritiesBelongs to the beclin family.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
- Information by UniProt
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
DAB stained, formalin-fixed paraffin-embedded human pancreas tissue stained for Beclin 1 using ab231341 at 30 µg/mL in immunohistochemical analysis.
All lanes : Anti-Beclin 1 antibody (ab231341) at 2 µg/ml
Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Porcine brain tissue
DAB stained, formalin-fixed paraffin-embedded human stomach tissue stained for Beclin 1 using ab231341 at 30 µg/mL in immunohistochemical analysis.
DAB stained, formalin-fixed paraffin-embedded human cerebrum tissue stained for Beclin 1 using ab231341 at 30 µg/mL in immunohistochemical analysis.
Anti-Beclin 1 antibody (ab231341) at 2 µg/ml + Recombinant human Beclin 1 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231341 has not yet been referenced specifically in any publications.