Product nameAnti-Beclin 1 antibody
See all Beclin 1 primary antibodies
DescriptionRabbit polyclonal to Beclin 1
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human Beclin 1 aa 50-100. The exact sequence is proprietary. NP_003757.1
Database link: Q14457
- IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPlays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Sequence similaritiesBelongs to the beclin family.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
- Information by UniProt
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
Beclin 1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264299 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Beclin 1 antibody at 1 µg/ml.
Lane 1: ab264299 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 10 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264299 has not yet been referenced specifically in any publications.