Overview

  • Product name
  • Description
    Rabbit polyclonal to Beclin 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human Beclin 1 (NP_003757.1)

  • Positive control
    • HeLa, 293T or NIH3T3 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab92389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 52 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the beclin family.
    • Cellular localization
      Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • APG6 antibody
      • ATG 6 antibody
      • ATG6 antibody
      • ATG6 autophagy related 6 homolog antibody
      • Bcl-2-interacting protein beclin antibody
      • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
      • Beclin 1 autophagy related antibody
      • Beclin-1 antibody
      • Beclin1 antibody
      • BECN 1 antibody
      • Becn1 antibody
      • BECN1_HUMAN antibody
      • Coiled coil myosin like BCL2 interacting protein antibody
      • Coiled-coil myosin-like BCL2-interacting protein antibody
      • GT197 antibody
      • Protein GT197 antibody
      • VPS 30 antibody
      • VPS30 antibody
      see all

    Images

    • All lanes : Anti-Beclin 1 antibody (ab92389)

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 52 kDa


      Exposure time: 3 minutes
    • Sample: HeLa whole cell lysate (1mg for IP, 20% of IP loaded).
      Antibodies: ab92389 used for WB 1µg/ml (lane 1) and used for IP at 3µg/mg lysate. Lane 2: control IgG.
      Detection: chemiluminescence with exposure time of 10 seconds.

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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