Overview

  • Product name

    Anti-Beclin 1 antibody [OTI4A10]
    See all Beclin 1 primary antibodies
  • Description

    Mouse monoclonal [OTI4A10] to Beclin 1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Beclin 1. Produced in HEK-293T cells. NP_003757
    Database link: Q14457

  • Positive control

    • WB: Beclin 1 transfected HEK-293T cell lysate. ICC/IF: COS-7 cells transiently transfected with Beclin 1. FC: HEK-293T cells transfected with Beclin 1.
  • General notes

    The clone number has been updated from 4A10 to OTI4A10, both clone numbers name the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab118148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 52 kDa.
ICC/IF 1/100.
Flow Cyt 1/100.

Target

  • Function

    Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the beclin family.
  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • APG6 antibody
    • ATG 6 antibody
    • ATG6 antibody
    • ATG6 autophagy related 6 homolog antibody
    • Bcl-2-interacting protein beclin antibody
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
    • Beclin 1 autophagy related antibody
    • Beclin-1 antibody
    • Beclin1 antibody
    • BECN 1 antibody
    • Becn1 antibody
    • BECN1_HUMAN antibody
    • Coiled coil myosin like BCL2 interacting protein antibody
    • Coiled-coil myosin-like BCL2-interacting protein antibody
    • GT197 antibody
    • Protein GT197 antibody
    • VPS 30 antibody
    • VPS30 antibody
    see all

Images

  • All lanes : Anti-Beclin 1 antibody [OTI4A10] (ab118148) at 1/2000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control
    Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY Beclin 1

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 52 kDa

  • pCMV6-ENTRY Beclin 1 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for Beclin 1 using ab118148 at a 1/100 dilution in ICC/IF.

  • HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either Beclin 1 (Red) or empty vector control plasmid (Blue) were immunostained by ab118148, and then analyzed by flow cytometry.

References

This product has been referenced in:

  • Blaszczak W  et al. Fucoidan Exerts Anticancer Effects Against Head and Neck Squamous Cell Carcinoma In Vitro. Molecules 23:N/A (2018). Read more (PubMed: 30545161) »
See 1 Publication for this product

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