Overview

  • Product name
    Anti-BEN Domain Containing Protein 5 antibody
    See all BEN Domain Containing Protein 5 primary antibodies
  • Description
    Goat polyclonal to BEN Domain Containing Protein 5
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human BEN Domain Containing Protein 5 aa 408-421 (C terminal).
    Sequence:

    CKNEERREAKYNLQ


    Database link: NP_078879.2

  • Positive control
    • Daudi cell lysate; Human tonsil and spleen lysates; mouse spleen lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab177244 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

Images

  • All lanes : Anti-BEN Domain Containing Protein 5 antibody (ab177244) at 0.3 µg/ml

    Lane 1 : Daudi cell lysate (in RIPA buffer)
    Lane 2 : Human tonsil lysate (in RIPA buffer)
    Lane 3 : Human spleen lysate (in RIPA buffer)
    Lane 4 : Mouse spleen lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    Primary incubation was 1 hour

References

ab177244 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreatic Cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Specification
Pancreatic Cells
Blocking step
CAS Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 23 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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