Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)
![Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)](https://www.abcam.com/ps/products/2/ab2182/Images/ab2182-5450-anti-bestrophin-antibody-e6-6-western-blot.jpg "Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)")
Key features and details
- Mouse monoclonal [E6-6] to Bestrophin/BEST1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Bestrophin/BEST1 antibody [E6-6]
See all Bestrophin/BEST1 primary antibodies -
Description
Mouse monoclonal [E6-6] to Bestrophin/BEST1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Bestrophin/BEST1 aa 568-585 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:KDHMDPYWALENRDEAHS
Database link: O76090 -
Positive control
- IHC-Fr: Pig retinal pigment epithelium tissue. ICC/IF: Bovine retinal pigment epithelium (RPE). WB: Human RPE cell lysate.
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General notes
This product was previously labelled as Bestrophin
This product has switched from ascites to TCS on 09th September 2020.
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Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: Ascites -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
E6-6 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Non human primates
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000.
|
| Notes |
|---|
|
WB
1/1000. |
Target
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Function
Forms calcium-sensitive chloride channels. Highly permeable to bicarbonate. -
Tissue specificity
Predominantly expressed in the basolateral membrane of the retinal pigment epithelium. -
Involvement in disease
Defects in BEST1 are the cause of vitelliform macular dystrophy type 2 (VMD2) [MIM:153700]; also known as Best macular dystrophy (BMD). VMD2 is an autosomal dominant form of macular degeneration that usually begins in childhood or adolescence. VMD2 is characterized by typical 'egg-yolk' macular lesions due to abnormal accumulation of lipofuscin within and beneath the retinal pigment epithelium cells. Progression of the disease leads to destruction of the retinal pigment epithelium and vision loss.
Defects in BEST1 are the cause of retinitis pigmentosa type 50 (RP50) [MIM:613194]. A retinal dystrophy belonging to the group of pigmentary retinopathies. RP is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Defects in BEST1 are a cause of adult-onset vitelliform macular dystrophy (AVMD) [MIM:608161]. AVMD is a rare autosomal dominant disorder with incomplete penetrance and highly variable expression. Patients usually become symptomatic in the fourth or fifth decade of life with a protracted disease of decreased visual acuity.
Defects in BEST1 are the cause of bestrophinopathy autosomal recessive (ARB) [MIM:611809]. A retinopathy characterized by central visual loss, an absent electro-oculogram light rise, and a reduced electroretinogram.
Defects in BEST1 are the cause of vitreoretinochoroidopathy autosomal dominant (ADVIRC) [MIM:193220]. A disorder characterized by vitreoretinochoroidal dystrophy. The clinical presentation is variable and may be associated with cataract, nanophthalmos, microcornea, shallow anterior chamber, and glaucoma. -
Sequence similarities
Belongs to the bestrophin family. -
Post-translational
modificationsPhosphorylated by PP2A. -
Cellular localization
Cell membrane. Basolateral cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7439 Human
- Omim: 607854 Human
- SwissProt: O76090 Human
- Unigene: 524910 Human
- Unigene: 712676 Human
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Alternative names
- ARB antibody
- BEST 1 antibody
- BEST antibody
see all
Images
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Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) at 1/1000 dilution + Human RPE cell lysate
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![Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)](https://www.abcam.com/ps/products/2/ab2182/Images/ab2182-5452-anti-bestrophin-antibody-e6-6-western-blot.jpg)
Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)This image is courtesy of an Abreview submitted by Dr Vladimir MilenkovicAll lanes : Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) at 1/1000 dilution
Lane 1 : Human RPE, retinal pigment epithelial cell lysate
Lane 2 : Non transfected HEK 293 cell extract
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-mouse
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 67 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesThe primary antobody was diluted in PBS/Tween/5%Milk and incubated for 1.5 hours at 25°C.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab2182 has been referenced in 22 publications.
- Hazim RA et al. Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide. Exp Eye Res 179:18-24 (2019). PubMed: 30336127
- Shen H et al. A novel xeno-free culture system for human retinal pigment epithelium cells. Int J Ophthalmol 12:563-570 (2019). PubMed: 31024807
- Wood SR et al. A Quantitative Chloride Channel Conductance Assay for Efficacy Testing of AAV.BEST1. Hum Gene Ther Methods 30:44-52 (2019). PubMed: 30963787
- Bai X et al. Generation of an integration-free induced pluripotent stem cell line (FDEENTi003-A) from a patient with pathological myopia. Stem Cell Res 39:101495 (2019). PubMed: 31376721
- Buskin A et al. Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa. Nat Commun 9:4234 (2018). PubMed: 30315276