Anti-beta 1 Adrenergic Receptor antibody (ab3442)
- Datasheet
- Specific References (13)
- Protocols
Overview
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Product nameAnti-beta 1 Adrenergic Receptor antibody
See all beta 1 Adrenergic Receptor primary antibodies -
DescriptionRabbit polyclonal to beta 1 Adrenergic Receptor
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IP, IHC-FoFr, ICC, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Cat, Human, Non human primates, African green monkey, Prairie vole
Predicted to work with: Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Mouse beta 1 Adrenergic Receptor aa 394-408.
Sequence:HGDRPRASGCLARAG
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
PurityWhole antiserum
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Primary antibody notesAdrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism. In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function. Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system. Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab3442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 50.5 kDa (predicted molecular weight: 50 kDa). | |
| ICC/IF | 1/100. Immunofluorescence staining of B1AR in mouse kidney distal tubule yields a pattern consistent with plasma membrane staining. | |
| IP | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| ICC | 1/5000. | |
| IHC-P | 1/200. | |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.
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DomainThe PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.
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Post-translational
modificationsHomologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. -
Cellular localizationCell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
- Information by UniProt
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Database links
- Entrez Gene: 493971 Cat
- Entrez Gene: 153 Human
- Entrez Gene: 11554 Mouse
- Entrez Gene: 24925 Rat
- Omim: 109630 Human
- SwissProt: Q9TST6 Cat
- SwissProt: P08588 Human
- SwissProt: P34971 Mouse
see all -
Alternative names
- ADR B1 antibody
- ADRB 1 antibody
- ADRB1 antibody
see all
Images
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Western blot - Anti-beta 1 Adrenergic Receptor antibody (ab3442)All lanes : Anti-beta 1 Adrenergic Receptor antibody (ab3442) at 1/1000 dilution
All lanes :
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Western blot analysis of ADR-Beta-1 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with ab3442 at a dilution of 1/1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1/20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed. -
Immunocytochemistry/ Immunofluorescence - Anti-beta 1 Adrenergic Receptor antibody (ab3442)ab3442 at 1/100 dilution staining beta 1 Adrenergic Receptor in untreated HeLa cells by Immunocytochemistry/ Immunofluorescence. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1, at a dilution of 1/100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488-conjugated goat-anti-rabbit secondary antibody at a dilution of 1/400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye. -
Flow Cytometry - Anti-beta 1 Adrenergic Receptor antibody (ab3442)This image is courtesy of an anonymous Abreviewab3442 staining the beta 1 Adrenergic Receptor in mouse bone marrow cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.5% BSA in 2mM EDTA/PBS. The sample was incubated with the primary antibody (1/100) for 20 minutes at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Gating Strategy: Blood cells. -
Immunocytochemistry - Anti-beta 1 Adrenergic Receptor antibody (ab3442)Immunolocalization of B1AR in mouse kidney distal tubule using ab3442.
Protocols
References
This product has been referenced in:
- Sun F et al. Stage-dependent changes of ß2-adrenergic receptor signaling in right ventricular remodeling in monocrotaline-induced pulmonary arterial hypertension. Int J Mol Med 41:2493-2504 (2018). Read more (PubMed: 29393391) »
- Huang Y et al. Downregulation of the ß1 adrenergic receptor in the myocardium results in insensitivity to metoprolol and reduces blood pressure in spontaneously hypertensive rats. Mol Med Rep 15:703-711 (2017). Read more (PubMed: 28000860) »
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