Anti-beta 1 Adrenergic Receptor antibody (ab3442)
Key features and details
- Rabbit polyclonal to beta 1 Adrenergic Receptor
- Suitable for: ICC, WB
- Reacts with: Mouse, Cat, Human, Non human primates
- Isotype: IgG
Overview
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Product name
Anti-beta 1 Adrenergic Receptor antibody
See all beta 1 Adrenergic Receptor primary antibodies -
Description
Rabbit polyclonal to beta 1 Adrenergic Receptor -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC MouseHuman -
Immunogen
Synthetic peptide corresponding to Mouse beta 1 Adrenergic Receptor aa 394-408.
Sequence:HGDRPRASGCLARAG
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Primary antibody notes
Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism. In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function. Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system. Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC |
Mouse
Human
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All applications |
Rhesus monkey
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Application | Abreviews | Notes |
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ICC | (1) |
1/100.
Immunofluorescence staining of B1AR in mouse kidney distal tubule yields a pattern consistent with plasma membrane staining. |
WB | (1) |
1/1000. Detects a band of approximately 50.5 kDa (predicted molecular weight: 50 kDa).
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Notes |
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ICC
1/100. Immunofluorescence staining of B1AR in mouse kidney distal tubule yields a pattern consistent with plasma membrane staining. |
WB
1/1000. Detects a band of approximately 50.5 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily. -
Domain
The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor. -
Post-translational
modificationsHomologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. -
Cellular localization
Cell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression. - Information by UniProt
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Database links
- Entrez Gene: 493971 Cat
- Entrez Gene: 153 Human
- Entrez Gene: 11554 Mouse
- Omim: 109630 Human
- SwissProt: Q9TST6 Cat
- SwissProt: P08588 Human
- SwissProt: P34971 Mouse
- SwissProt: P47899 Rhesus monkey
see all -
Alternative names
- ADR B1 antibody
- ADRB 1 antibody
- ADRB1 antibody
see all
Images
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All lanes : Anti-beta 1 Adrenergic Receptor antibody (ab3442) at 1/1000 dilution
All lanes :
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Western blot analysis of ADR-Beta-1 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with ab3442 at a dilution of 1/1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1/20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed. -
ab3442 at 1/100 dilution staining beta 1 Adrenergic Receptor in untreated HeLa cells by Immunocytochemistry/ Immunofluorescence. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1, at a dilution of 1/100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488-conjugated goat-anti-rabbit secondary antibody at a dilution of 1/400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye.
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Immunolocalization of B1AR in mouse kidney distal tubule using ab3442.
Protocols
References (35)
ab3442 has been referenced in 35 publications.
- Dang S et al. Blockade of ß-adrenergic signaling suppresses inflammasome and alleviates cardiac fibrosis. Ann Transl Med 8:127 (2020). PubMed: 32175420
- Chen SY et al. Decreased basal and stimulated salivary parameters by histopathological lesions and secretory dysfunction of parotid and submandibular glands in rats with type 2 diabetes. Exp Ther Med 19:2707-2719 (2020). PubMed: 32256753
- Renard HF et al. Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166. Nat Commun 11:1457 (2020). PubMed: 32193381
- Sun R et al. Nanodiscs Incorporating Native ß1 Adrenergic Receptor as a Novel Approach for the Detection of Pathological Autoantibodies in Patients with Dilated Cardiomyopathy. J Appl Lab Med 4:391-403 (2019). PubMed: 31659076
- Ghosh A et al. An experimental model of Braak's pretangle proposal for the origin of Alzheimer's disease: the role of locus coeruleus in early symptom development. Alzheimers Res Ther 11:59 (2019). PubMed: 31266535