Anti-beta 1 Adrenergic Receptor antibody (ab77189)
Key features and details
- Goat polyclonal to beta 1 Adrenergic Receptor
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta 1 Adrenergic Receptor antibody
See all beta 1 Adrenergic Receptor primary antibodies -
Description
Goat polyclonal to beta 1 Adrenergic Receptor -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Cat, Rhesus monkey, Gerbil -
Immunogen
Synthetic peptide corresponding to Human beta 1 Adrenergic Receptor (internal sequence).
Sequence:ESDEARRCYNDPK
Database link: NP_000675.1 -
Positive control
- IHC-P: Human heart tissue. ICC/IF: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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IHC-P | (1) |
Use a concentration of 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily. -
Domain
The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor. -
Post-translational
modificationsHomologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. -
Cellular localization
Cell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression. - Information by UniProt
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Database links
- Entrez Gene: 493971 Cat
- Entrez Gene: 153 Human
- Entrez Gene: 11554 Mouse
- Entrez Gene: 24925 Rat
- Omim: 109630 Human
- SwissProt: Q9TST6 Cat
- SwissProt: O70430 Gerbil
- SwissProt: P08588 Human
see all -
Alternative names
- ADR B1 antibody
- ADRB 1 antibody
- ADRB1 antibody
see all
Images
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Immmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling beta 1 Adrenergic Receptor with ab77189 at 6 µg/mL. Heat mediated antigen retrieval was performed with citrate buffer pH 6. HRP-staining.
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Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab77189 has been referenced in 9 publications.
- Choi S et al. Aging Alters the Formation and Functionality of Signaling Microdomains Between L-type Calcium Channels and β2-Adrenergic Receptors in Cardiac Pacemaker Cells. Front Physiol 13:805909 (2022). PubMed: 35514336
- Sun H et al. Tragus Nerve Stimulation Suppresses Post-Infarction Ventricular Arrhythmia by Modulating Autonomic Activity and Heterogeneities of Cardiac Receptor Distribution. Med Sci Monit 26:e922277 (2020). PubMed: 32447339
- Wang ZM et al. Aging Blunts Sympathetic Neuron Regulation of Motoneurons Synaptic Vesicle Release Mediated by ß1- and a2B-Adrenergic Receptors in Geriatric Mice. J Gerontol A Biol Sci Med Sci 75:1473-1480 (2020). PubMed: 31956900
- Xiong L et al. Targeted ablation of cardiac sympathetic neurons attenuates adverse postinfarction remodelling and left ventricular dysfunction. Exp Physiol 103:1221-1229 (2018). PubMed: 29928790
- Phillips RJ et al. Infantile haemangiomas that failed treatment with propranolol: clinical and histopathological features. J Paediatr Child Health 50:619-25 (2014). PubMed: 24905852
- O'Leary AP et al. Beta-Adrenoceptor Activation Reduces Both Dermal Microvascular Endothelial Cell Migration Via a cAMP-Dependent Mechanism and Wound Angiogenesis. J Cell Physiol N/A:N/A (2014). WB ; Human . PubMed: 24986762
- Famulla S et al. Differentiation of human adipocytes at physiological oxygen levels results in increased adiponectin secretion and isoproterenol-stimulated lipolysis. Adipocyte 1:132-181 (2012). Human . PubMed: 23700522
- Schuller HM et al. Regulation of pancreatic cancer by neuropsychological stress responses: a novel target for intervention. Carcinogenesis 33:191-6 (2012). WB ; Human . PubMed: 22072614
- Wallukat G et al. Distinct patterns of autoantibodies against G-protein-coupled receptors in Chagas' cardiomyopathy and megacolon. Their potential impact for early risk assessment in asymptomatic Chagas' patients. J Am Coll Cardiol 55:463-8 (2010). Rat . PubMed: 20117461