Anti-beta 1 Adrenergic Receptor antibody (ab77189)

Reviews (2)Q&A (11)References (4)

Overview

  • Product name
    Anti-beta 1 Adrenergic Receptor antibody
    See all beta 1 Adrenergic Receptor primary antibodies
  • Description
    Goat polyclonal to beta 1 Adrenergic Receptor
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human
    Predicted to work with: Mouse, Guinea pig, Cat, Rhesus monkey, Gerbil
  • Immunogen

    Synthetic peptide:

    ESDEARRCYNDPK

    from the internal region of Human beta 1 Adrenergic Receptor

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.
  • Domain
    The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.
  • Post-translational
    modifications
    Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
  • Cellular localization
    Cell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ADR B1 antibody
    • ADRB 1 antibody
    • ADRB1 antibody
    • ADRB1_HUMAN antibody
    • ADRB1R antibody
    • Adrenergic beta 1 receptor antibody
    • Adrenoceptor beta 1 antibody
    • B1AR antibody
    • Beta 1 adrenoceptor antibody
    • Beta 1 adrenoreceptor antibody
    • Beta-1 adrenergic receptor antibody
    • Beta-1 adrenoceptor antibody
    • Beta-1 adrenoreceptor antibody
    • BETA1AR antibody
    • RHR antibody
    see all

Images

  • Western blot - Anti-beta 1 Adrenergic Receptor antibody (ab77189)
    Western blot - Anti-beta 1 Adrenergic Receptor antibody (ab77189)
    Anti-beta 1 Adrenergic Receptor antibody (ab77189) at 2 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg

    Predicted band size: 51 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Primary incubation time: 1 hour

    Detected by chemiluminescence

References

This product has been referenced in:
  • O'Leary AP  et al. Beta-Adrenoceptor Activation Reduces Both Dermal Microvascular Endothelial Cell Migration Via a cAMP-Dependent Mechanism and Wound Angiogenesis. J Cell Physiol N/A:N/A (2014). WB ; Human . Read more (PubMed: 24986762) »
  • Famulla S  et al. Differentiation of human adipocytes at physiological oxygen levels results in increased adiponectin secretion and isoproterenol-stimulated lipolysis. Adipocyte 1:132-181 (2012). Human . Read more (PubMed: 23700522) »
See all 4 Publications for this product

Publishing research using ab77189? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 13 Abreviews or Q&A

ELISA abreview for Anti-beta 1 Adrenergic Receptor antibody

 Excellent
Abreviews
Application
ELISA
Sample
Human Recombinant protein (Synthetised peptide from human beta 1 adrenergic r)
Specification
Synthetised peptide from human beta 1 adrenergic r
Type
Other
Read More

Abcam user community

Verified customer

Submitted Aug 03 2015

Western blot abreview for Anti-beta 1 Adrenergic Receptor antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Dog Tissue lysate - whole (heart)
Specification
heart
Blocking step
(agent) for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Apr 14 2014

Question

I would like to test ab77189 in western blotting/flow.

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Answer


I am very pleased to hear you would like to accept our offer and testab77189 inwestern blot/flow.This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forwestern blotand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

The customer, Isil Ozakca, would like a free of charge replacement. The order number (you asked in following e-mail) is 1096052.
Could you please sent the antibody with KIMERA's next shipment?

Thank you in advance.

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Answer

Thank you for confirming the order number.

ab77189, one vial has been added to your next order 1151187.

Thanks you very much for your cooperation.

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Question

Actually I have no idea about “purchase order number”, won’t you send me the requested antibody by your distributor in Turkey?

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Answer

I am sorry I can't send you the antibody without receiving the purchase order number. You need to contact our distributor Kimera for this. They can contact us on your behalf with purchase order number. So please cotnact them and discuss.

Thanks!

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Question

The customer asks a new vial ab77189 from a different batch (the antibody's lot was GR55557-1) as a free of charge. Could you please add the antibody to our current order?

P.S.: We offer technical support to Turkish customers; but some customers contact directly with you sometimes (like the following e-mail).
We kindly ask for a information about e-mails you send to the Turkish customer. We will appreciate you sent your reply to us also as Cc when a Turkish customer contact directly with you (about technical support or testing discount code etc).

Thank you for your understanding.

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Answer

Could you provide the purchase order number for us to send the requested replacement antibodies?

Thanks!

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Question

The size of non-specific bands are usually smaller than predicted size (<51 kDa). You can find the images as attached. I sent several images in which the experimental conditions were changed each of them. Especially in B and C, the non-specific bands are so strong. In the image A, the samples are membrane fraction (but I have no positive control to check this fraction is the right one), and it seems the density of non-specific bands were weaker compared to whole tissue lysate.
Best,

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Answer

Thank you for getting back to me.

I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

I could offer either a new vial as a free of charge replacement from a different batch or a credit note which your customer could use for future purchase. Please discuss this offer with your customer and let me know how to proceed with this enquiry.

I look forward to hearing from you soon.

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Question

Product code: 77189
Lot number: GR55557-1
Inquiry: 1) Abcam product code ab77189 2) Abcam order reference number or product batch number GR55557-1 3) Description of the problem No band detection at predicted size and also non-specific bands are observed 4) Sample preparation: Type of sample (whole cell lysates, fraction, recombinant protein…) whole tissue lysate and membrane fraction Lysis buffer RIPA buffer for whole tissue lysate, a buffer containing tris HCl, sucrose, DTT and protease inhibitor cocktail for membrane fraction Protease inhibitors: Protease inhibitor cocktail for Sigma Phosphatase inhibitors Sodium orthovanadate Reducing agent DTT Boiling for ≥5 min? At 60C for 15 min Protein loaded ug/lane or cells/lane 20-50 microgram Positive control No Negative control No 5) Percentage of gel 10% Type of membrane PVDF Protein transfer verified Prestained protein ladder and Coomassie Blue staining of the gel after transfer Blocking agent and concentration 5% BSA in TBS containing 0,1% Tween 20 Blocking time 1,5-2 hours Blocking temperature room temperture 6) Primary antibody (If more than one was used, describe in “additional notes”) : Concentration or dilution 1/1000 Diluent buffer 5% BSA in TBS containing 0,1% Tween 20 Incubation time overnight (14-16 hours) Incubation temperature: 4C 7) Secondary antibody: Species: rabbit (ab97100) Reacts against: goat Concentration or dilution 1/10000 Diluent buffer 5% BSA in TBS containing 0,1% Tween 20 Incubation time 2 hours Incubation temperature: 4C Fluorochrome or enzyme conjugate: 8) Washing after primary and secondary antibodies: Buffer 5% BSA in TBS containing 0,1% Tween 20 Number of washes 4X15 minutes 9) Detection method ECL (Pierce) 10) How many times have you run this staining? (approximately 10 times, I changed different parameters/conditions each time, but none worked. Do you obtain the same results every time? Yes, for membrane fraction the non-specific band intensity decreased compared to whole cell lysate. What steps have you altered to try and optimize the use of this antibody? Type of sample : Whole tissue lysate vs membrane fraction Denaturation: agents (beta-mercaptoethanol vs DTT), boiling conditions (100C for 4 minutes vs 37C for 20 min vs 60C for 15 min) Loading concentrations (from 1 to 100 microgram protein per lane) Blocking conditions: agent (non-fat dry milk vs BSA), duration (from 1 hour to 4 hours). Primary antibody: dilution (from 1/2000 to 1/1000) Secondary antibody: dilution (from 1/20000 to 1/10000) P.S.: I sent the images of blots in my last e-mail.

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Answer

Thank you for providing some information.

Could you please specify what size are the non-specific bands?

Are they lower or higher than the expected size (51 kDa)?

Could you attach an image (saved as a jpeg file) to your e-mail so that I can have a look at the bands.

I look forward to hearing from you soon.

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Question

Lot number: GR55557-1
Inquiry: The images were represented the non-specific blots for beta1-adrenoceptor antibody. For (A), the upper signals were SERCA to control antibody-independent part of WB protocol. Other images were belonged to beta1-adrenoceptor. Your suggestions are not helped, because I have not seen any signal (band of interest or non-specific).

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Answer

Thank you for your recent e-mail and for sending the Western blot image to our Scientific Support Team.

My colleague is out of office for two weeks and he has asked me to look after her customers during her absence.

Could you provide some further details of the protocol used and complete the following form (attached as a word document).

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

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Question

Inquiry: I cannot detect beta1-adrenoceptor protein at predicted size. I followed your suggestions (using fresh protease inhibitors, incubating with sample buffer at 60C for 20 minutes, blocking the membrane with 5% BSA instead of 5% non-fat dry milk, incubating the primary antibody as mentioned), but still no results. I also loaded 70 and 100 microgram protein per lane, but it didn't help too.

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Answer

I am sorry to hear that you are experiencing problems again.

Could you summarize the results? Do you observe high back ground or no staining. I presuming you are not observing band of interest at different size so could you provide image of staining which would help me to understand what could have gone wrong with this product?

Thanks!

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1-10 of 13 Abreviews or Q&A

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