Anti-beta 1 Adrenergic Receptor antibody (ab77189)


  • Product name
    Anti-beta 1 Adrenergic Receptor antibody
    See all beta 1 Adrenergic Receptor primary antibodies
  • Description
    Goat polyclonal to beta 1 Adrenergic Receptor
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human
    Predicted to work with: Mouse, Guinea pig, Cat, Rhesus monkey, Gerbil
  • Immunogen

    Synthetic peptide:


    from the internal region of Human beta 1 Adrenergic Receptor

  • Positive control
    • Human heart lysate



Our Abpromise guarantee covers the use of ab77189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
IHC-P Use at an assay dependent concentration.


  • Function
    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.
  • Domain
    The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.
  • Post-translational
    Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
  • Cellular localization
    Cell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADR B1 antibody
    • ADRB 1 antibody
    • ADRB1 antibody
    • ADRB1_HUMAN antibody
    • ADRB1R antibody
    • Adrenergic beta 1 receptor antibody
    • Adrenoceptor beta 1 antibody
    • B1AR antibody
    • Beta 1 adrenoceptor antibody
    • Beta 1 adrenoreceptor antibody
    • Beta-1 adrenergic receptor antibody
    • Beta-1 adrenoceptor antibody
    • Beta-1 adrenoreceptor antibody
    • BETA1AR antibody
    • RHR antibody
    see all


  • Anti-beta 1 Adrenergic Receptor antibody (ab77189) at 2 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg

    Predicted band size: 51 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)

    Primary incubation time: 1 hour

    Detected by chemiluminescence


This product has been referenced in:
  • O'Leary AP  et al. Beta-Adrenoceptor Activation Reduces Both Dermal Microvascular Endothelial Cell Migration Via a cAMP-Dependent Mechanism and Wound Angiogenesis. J Cell Physiol N/A:N/A (2014). WB ; Human . Read more (PubMed: 24986762) »
  • Famulla S  et al. Differentiation of human adipocytes at physiological oxygen levels results in increased adiponectin secretion and isoproterenol-stimulated lipolysis. Adipocyte 1:132-181 (2012). Human . Read more (PubMed: 23700522) »

See all 4 Publications for this product

Customer reviews and Q&As

Human Recombinant protein (Synthetised peptide from human beta 1 adrenergic r)
Synthetised peptide from human beta 1 adrenergic r

Abcam user community

Verified customer

Submitted Aug 03 2015

Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Dog Tissue lysate - whole (heart)
Blocking step
(agent) for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 14 2014

I am very pleased to hear you would like to accept our offer and testab77189 inwestern blot/flow.This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forwestern blotand includ...

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Thank you for confirming the order number.

ab77189, one vial has been added to your next order 1151187.

Thanks you very much for your cooperation.

I am sorry I can't send you the antibody without receiving the purchase order number. You need to contact our distributor Kimera for this. They can contact us on your behalf with purchase order number. So please cotnact them and discuss.


Could you provide the purchase order number for us to send the requested replacement antibodies?


Thank you for getting back to me.

I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the ap...

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Thank you for providing some information.

Could you please specify what size are the non-specific bands?

Are they lower or higher than the expected size (51 kDa)?

Could you attach an image (saved as a jpeg file) to your e-...

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Thank you for your recent e-mail and for sending the Western blot image to our Scientific Support Team.

My colleague is out of office for two weeks and he has asked me to look after her customers during her absence.

Could you provid...

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I am sorry to hear that you are experiencing problems again.

Could you summarize the results? Do you observe high back ground or no staining. I presuming you are not observing band of interest at different size so could you provide image of st...

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