Overview

  • Product name
    Anti-beta 1 Adrenergic Receptor antibody
    See all beta 1 Adrenergic Receptor primary antibodies
  • Description
    Rabbit polyclonal to beta 1 Adrenergic Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Sheep, Cow, Cat, Human, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse beta 1 Adrenergic Receptor.

    Read Abcam's proprietary immunogen policy (Peptide available as ab97424.)

  • Positive control
    • This antibody gave a positive signal in Mouse Spinal cord tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.
  • Domain
    The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.
  • Post-translational
    modifications
    Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
  • Cellular localization
    Cell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADR B1 antibody
    • ADRB 1 antibody
    • ADRB1 antibody
    • ADRB1_HUMAN antibody
    • ADRB1R antibody
    • Adrenergic beta 1 receptor antibody
    • Adrenoceptor beta 1 antibody
    • B1AR antibody
    • Beta 1 adrenoceptor antibody
    • Beta 1 adrenoreceptor antibody
    • Beta-1 adrenergic receptor antibody
    • Beta-1 adrenoceptor antibody
    • Beta-1 adrenoreceptor antibody
    • BETA1AR antibody
    • RHR antibody
    see all

Images

  • Anti-beta 1 Adrenergic Receptor antibody (ab85037) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    Mouse Beta-1 adrenergic receptor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab85037 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85037, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Shimizu I  et al. Vascular rarefaction mediates whitening of brown fat in obesity. J Clin Invest 124:2099-112 (2014). WB ; Mouse . Read more (PubMed: 24713652) »
  • Sugatani J  et al. Antiobese function of platelet-activating factor: increased adiposity in platelet-activating factor receptor-deficient mice with age. FASEB J 28:440-52 (2014). Read more (PubMed: 24100020) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Sheep Tissue lysate - whole (Adipose Fat)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
100 µg
Specification
Adipose Fat
Blocking step
Licor as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C

Kanchan Kaushal

Verified customer

Submitted Aug 01 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary culture of cortical rat astrocytes)
Permeabilization
Yes - 0.1 % Triton X-100, 10 min
Specification
primary culture of cortical rat astrocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 23 2016

Answer

Thank you for contacting us.

The host for ab40834 is Goat; you would need an anti goat secondary antibody e.g. ab97100

The host for ab85037 is rabbit; you would need anti rabbit antibody e.g. ab6721

Common secondary can not be used for both of these antibodies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

Thank you very much for your interest our products.

I have to confirm that we do not have any Anti-beta 1 Adrenergic Receptor antibodies that are tested and guaranteed for bovine samples.

But I am happy to also confirm that the immunogen of ab85037 shows a 100% homology with bovine beta 1 Adrenergic Receptor and therefore it is very likely that this antibody will also bind to bovine beta 1 Adrenergic Receptor.


To our knowledge, ab85037 has not been experimentally tested in bovine samples. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Neonatal cardiomyocytes)
Specification
Neonatal cardiomyocytes
Preparation
Cell harvesting/tissue preparation method: Scraper
Sample buffer: 0,5 mM EDTA/PBS
Permeabilization
No
Gating Strategy
Total cardiomyocytes

Abcam user community

Verified customer

Submitted Jul 19 2012

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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