Product nameAnti-beta 1 Adrenergic Receptor antibody
See all beta 1 Adrenergic Receptor primary antibodies
DescriptionRabbit polyclonal to beta 1 Adrenergic Receptor
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Sheep, Cow, Cat, Human, Pig
- This antibody gave a positive signal in Mouse Spinal cord tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab85037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).|
FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.
DomainThe PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.
modificationsHomologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
Cellular localizationCell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
- Information by UniProt
- ADR B1 antibody
- ADRB 1 antibody
- ADRB1 antibody
Anti-beta 1 Adrenergic Receptor antibody (ab85037) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Mouse Beta-1 adrenergic receptor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ICC/IF image of ab85037 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85037, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Shimizu I et al. Vascular rarefaction mediates whitening of brown fat in obesity. J Clin Invest 124:2099-112 (2014). WB ; Mouse . Read more (PubMed: 24713652) »
- Sugatani J et al. Antiobese function of platelet-activating factor: increased adiposity in platelet-activating factor receptor-deficient mice with age. FASEB J 28:440-52 (2014). Read more (PubMed: 24100020) »