Product nameAnti-beta 1 Sodium Potassium ATPase antibody [464.8]
See all beta 1 Sodium Potassium ATPase primary antibodies
DescriptionMouse monoclonal [464.8] to beta 1 Sodium Potassium ATPase
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Rat, Cow, Dog, Human, Pig
Purified Na,K-ATPase from rabbit renal outermedulla
- ab8344 gave a positive signal in human, mouse and rat kidney tissue lysates. In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HepG2 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Contains 0.4M arginine
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab8344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|WB||1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).|
FunctionThis is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Tissue specificityFound in most tissues.
Sequence similaritiesBelongs to the X(+)/potassium ATPases subunit beta family.
- Information by UniProt
- Adenosinetriphosphatase antibody
- AT1B1_HUMAN antibody
- ATP 1B antibody
Anti-beta 1 Sodium Potassium ATPase antibody [464.8] (ab8344) at 1/500 dilution + Human kidney tissue lysate - total protein (ab30203) at 20 µg
Granzyme B overexpression 293T lysate (whole cell) (ab94040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Overlay histogram showing HepG2 cells stained with ab8344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8344, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Wen X et al. Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells. Eur J Oral Sci 122:21-6 (2014). Read more (PubMed: 24313748) »
- Lu Q et al. Ca²?/calmodulin-dependent protein kinase II contributes to hypoxic ischemic cell death in neonatal hippocampal slice cultures. PLoS One 8:e70750 (2013). WB ; Rat . Read more (PubMed: 23976956) »