Anti-ATP1B1 antibody [464.8] (ab8344)
Key features and details
- Mouse monoclonal [464.8] to ATP1B1
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG2a
Overview
-
Product name
Anti-ATP1B1 antibody [464.8]
See all ATP1B1 primary antibodies -
Description
Mouse monoclonal [464.8] to ATP1B1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Dog, Pig -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human kidney tissue lysates Flow Cyt (Intra): HepG2 cells.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Contains 0.4M arginine -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
464.8 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).
|
Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
---|
WB
1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa). |
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
-
Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. -
Tissue specificity
Found in most tissues. -
Sequence similarities
Belongs to the X(+)/potassium ATPases subunit beta family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 403966 Dog
- Entrez Gene: 481 Human
- Entrez Gene: 396898 Pig
- Entrez Gene: 25650 Rat
- Omim: 182330 Human
- SwissProt: P06583 Dog
- SwissProt: P05026 Human
- SwissProt: P05027 Pig
see all -
Alternative names
- Adenosinetriphosphatase antibody
- AT1B1_HUMAN antibody
- ATP 1B antibody
see all
Images
-
Anti-ATP1B1 antibody [464.8] (ab8344) at 1/500 dilution + Human kidney tissue lysate - total protein (ab30203) at 20 µg
Secondary
Granzyme B overexpression 293T lysate (whole cell) (ab94040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Overlay histogram showing HepG2 cells stained with ab8344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8344, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Datasheets and documents
-
SDS download
-
Datasheet download
References (12)
ab8344 has been referenced in 12 publications.
- Wen X et al. Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells. Eur J Oral Sci 122:21-6 (2014). PubMed: 24313748
- Lu Q et al. Ca²?/calmodulin-dependent protein kinase II contributes to hypoxic ischemic cell death in neonatal hippocampal slice cultures. PLoS One 8:e70750 (2013). WB ; Rat . PubMed: 23976956
- Mroz MS et al. Farnesoid X receptor agonists attenuate colonic epithelial secretory function and prevent experimental diarrhoea in vivo. Gut N/A:N/A (2013). WB ; Mouse . PubMed: 23916961
- Lu Q et al. Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures. Free Radic Biol Med 53:1139-51 (2012). WB . PubMed: 22728269
- Rafiqi FH et al. Role of the WNK-activated SPAK kinase in regulating blood pressure. EMBO Mol Med 2:63-75 (2010). PubMed: 20091762