• Product name

    Anti-beta 1 Spectrin antibody [EPR6259]
    See all beta 1 Spectrin primary antibodies
  • Description

    Rabbit monoclonal [EPR6259] to beta 1 Spectrin
  • Host species

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human beta 1 Spectrin aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Human red blood cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab129065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 246 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
    • Involvement in disease

      Defects in SPTB are the cause of elliptocytosis type 3 (EL3) [MIM:182870]. EL3 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
      Defects in SPTB are the cause of spherocytosis type 2 (SPH2) [MIM:182870]; also known as hereditary spherocytosis type 2 (HS2). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH2 is characterized by severe hemolytic anemia. Inheritance is autosomal dominant.
    • Sequence similarities

      Belongs to the spectrin family.
      Contains 2 CH (calponin-homology) domains.
      Contains 17 spectrin repeats.
    • Post-translational

      The first phosphorylation event occurs on Ser-2114, followed by Ser-2125, Ser-2123, Ser-2128, Ser-2117, and Thr-2110.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm > cell cortex.
    • Information by UniProt
    • Database links

    • Alternative names

      • Beta I spectrin antibody
      • Beta spectrin antibody
      • Beta-I spectrin antibody
      • EL3 antibody
      • erythrocyte antibody
      • HS2 antibody
      • HSpTB1 antibody
      • Membrane cytoskeletal protein antibody
      • Spectrin beta antibody
      • Spectrin beta chain antibody
      • Spectrin beta chain erythrocyte antibody
      • Spectrin beta erythrocytic (includes spherocytosis clinical type I) antibody
      • Spectrin beta erythrocytic antibody
      • SPH2 antibody
      • sptB antibody
      • SPTB1 antibody
      • SPTB1_HUMAN antibody
      see all


    • Anti-beta 1 Spectrin antibody [EPR6259] (ab129065) at 1/1000 dilution + Red blood cell lysate at 10 µg

      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 246 kDa


    This product has been referenced in:

    See all 2 Publications for this product

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