Recombinant Anti-beta 1 Spectrin antibody [EPR6259] (ab129065)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6259] to beta 1 Spectrin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-beta 1 Spectrin antibody [EPR6259]
See all beta 1 Spectrin primary antibodies -
Description
Rabbit monoclonal [EPR6259] to beta 1 Spectrin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human beta 1 Spectrin aa 300-400. The exact sequence is proprietary.
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Positive control
- Human red blood cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6259 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 246 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 246 kDa). |
Target
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Function
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. -
Involvement in disease
Defects in SPTB are the cause of elliptocytosis type 3 (EL3) [MIM:182870]. EL3 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
Defects in SPTB are the cause of spherocytosis type 2 (SPH2) [MIM:182870]; also known as hereditary spherocytosis type 2 (HS2). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH2 is characterized by severe hemolytic anemia. Inheritance is autosomal dominant. -
Sequence similarities
Belongs to the spectrin family.
Contains 2 CH (calponin-homology) domains.
Contains 17 spectrin repeats. -
Post-translational
modificationsThe first phosphorylation event occurs on Ser-2114, followed by Ser-2125, Ser-2123, Ser-2128, Ser-2117, and Thr-2110. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. - Information by UniProt
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Database links
- Entrez Gene: 6710 Human
- Omim: 182870 Human
- SwissProt: P11277 Human
- Unigene: 417303 Human
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Alternative names
- Beta I spectrin antibody
- Beta spectrin antibody
- Beta-I spectrin antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab129065 has been referenced in 2 publications.
- Hao L et al. Two novel ANK1 loss-of-function mutations in Chinese families with hereditary spherocytosis. J Cell Mol Med 23:4454-4463 (2019). PubMed: 31016877
- Sanford K et al. Attenuation of Red Blood Cell Storage Lesions with Vitamin C. Antioxidants (Basel) 6:N/A (2017). PubMed: 28704937