Overview

  • Product name
    Anti-beta 2 Adrenergic Receptor antibody
    See all beta 2 Adrenergic Receptor primary antibodies
  • Description
    Chicken polyclonal to beta 2 Adrenergic Receptor
  • Host species
    Chicken
  • Tested applications
    Suitable for: ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide:

    DFRIAFQELLC LRRSSLKAYGN GYSSNGNTGE QSGYHVEQEKE NKLLCEDLPGT EDFVGHQGTVP SDNIDSQGRNCS T

    , corresponding to amino acids 335-408 of Human beta 2 Adrenergic Receptor.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab13989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
ICC 1/1000.
WB 1/500. Predicted molecular weight: 46.5 kDa.

Target

  • Function
    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • Post-translational
    modifications
    Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all

Images

  • E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1/2000, Goat anti-IgY-HRP dilution: 1/1000. Colorimetric method for signal development. E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1/2000, Goat anti-IgY-HRP dilution: 1/1000. Colorimetric method for signal development.

References

This product has been referenced in:
  • Koryakina Y  et al. Effects of the ß-agonist, isoprenaline, on the down-regulation, functional responsiveness and trafficking of ß2-adrenergic receptors with N-terminal polymorphisms. Cell Biol Int 36:1171-83 (2012). Read more (PubMed: 22938397) »
  • Valentine CD & Haggie PM Confinement of ß(1)- and ß(2)-adrenergic receptors in the plasma membrane of cardiomyocyte-like H9c2 cells is mediated by selective interactions with PDZ domain and A-kinase anchoring proteins but not caveolae. Mol Biol Cell 22:2970-82 (2011). Read more (PubMed: 21680711) »
See all 3 Publications for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (astrocytes)
Permeabilization
Yes - TX100 0.05%
Specification
astrocytes
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 28 2016

Application
Western blot
Sample
Rat Cell lysate - whole cell (Astrocyte culture)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
1.7 µg
Specification
Astrocyte culture
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 2°C

Abcam user community

Verified customer

Submitted Sep 29 2015

Answer

Thank you very much for your reply which has been forwarded to me as my colleague #### is away from the office today.

To our knowledge, ab107217 has not been tested in ICC-IF. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk. I would therefore be pleased to offer you an AbTrial disocunt if you want to test ab107217 in ICC-IF.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

Read More

Answer

Thank you for contacting us and your interest in our products.

The Anti-beta 2 Adrenergic Receptor antibody (ab13989) has been shown to recognize the human and rat from of the protein, however, we have as yet not tested the antibody with mouse samples. As the homology between the immunogen used to raise the antibody and the mouse protein only shares 69%, I would not expect the antibody to cross react.

Are you just trying to find an antibody which is not raised in rabbit against beta 2 Adrenergic Receptor? If so, it may be worth considering the Chicken polyclonal https://www.abcam.com/beta-2-Adrenergic-Receptor-antibody-ab107217.htmlwhich has been shown to detect human, mouse and rat protein in Western blotting.

What application are you hoping to use the antibody in? We have as yet not tested it in any other application other than Western blotting. This does not mean that is would not work in other applications, simply that we have not tried it ourselves and would therefore not be able to guarantee its performance.

If you are not intending to use it in Western blotting you would be eligible for our testing discount scheme. This offer means that if you purchase ab107217 as normal, test the antibody with your samples in an application other than Western blotting and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab107217 off any product). More information on this offer can be found here:

www.abcam.com/collaborationdiscount

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab107217.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Answer

Thank you for your reply. A549 cells were recommended because they showed weak to no staining using a similar antibody.  Plus these are the results found in the Human Protein Atlas: http://www.proteinatlas.org/ENSG00000169252/cell/A-549 I'm not sure if I have a clear explanation as to why these stained well because fewer than 5% were shown to have a signal in the Human Protein Atlas, unless the antibody was binding to something non-specifically.  Did you run a no-primary control?   Let me know how the staining goes with the mouse keratinocytes.  I look forward to your reply.

Read More

Question
Answer

I have received word from the laboratory that the following cell lines should serve as a good positive control: HEL K-562 NB-4 U-937 Karpas-707 KM3 LP-1 U-698 D341 Med SHSy-5y AN3-CA EFO-21 SiHa SK-BR-3 NTERA-2 PC-3 A-431 HaCaT WM-115 U-2 OS U-2197 Hth 83 TIME As for a good negative control, I was not able to find a human cell line that would serve as a good negative control. Cell line A549 showed weak to no staining using a similar antibody (same immunogen sequence). I did do a homology check between the immunogen sequence used to develop the ab13989 antibody with that of the mouse ADRB2 protein and found only a 67% match by identity. Using a mouse cell line as a negative control would be best in this case. I would not expect there to be cross-reactivity. I hope you found this information to be helpful. Please do not hesitate to contact me if you have any additional questions.

Read More

Answer

Thank you for your reply and for providing the requested information. After seeing the data and your protocol, I would like to make some suggestions that may improve your results. One of my colleagues who has worked with chicken antibodies in the past has found them to be a bit "sticky" and suggested the following: Increase the amount of serum and blocking time. I see that for non-permeabilized cells you use 10% serum - I would advice increasing to 10% and incubating for 1-2 hours. Titrating the antibody concentrations - You appear to get a nice specific signal at 1:250. Continue to titrate that so that you still observe the specific signal and reduce the non-specific signal from the isotype control. Overall, the background is not too bad and this should be easy to titrate. I have contacted the laboratory regarding a negative control and will forward that information to you as soon as possible. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (HEK 293 cells stably expressing human beta2-AR)
Specification
HEK 293 cells stably expressing human beta2-AR
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1 Triton X -100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 12 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (HEK 293 cells transiently expressing rat beta2-AR)
Specification
HEK 293 cells transiently expressing rat beta2-AR
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 09 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary Cultures of Rat Airway Smooth Muscle and E)
Specification
Primary Cultures of Rat Airway Smooth Muscle and E
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C

Abcam user community

Verified customer

Submitted May 07 2008

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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