Overview

  • Product name
    Anti-beta 2 Adrenergic Receptor antibody
    See all beta 2 Adrenergic Receptor primary antibodies
  • Description
    Rabbit polyclonal to beta 2 Adrenergic Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Guinea pig, Hamster, Cat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Mouse beta 2 Adrenergic Receptor (N terminal). The exact sequence is proprietary.
    Database link: P18762

  • Positive control
    • Rat heart membrane extract, Rat brain stem, HEK293 cells expressing beta 2 Adrenergic Receptor, Lewis rat brain membranes

Properties

Applications

Our Abpromise guarantee covers the use of ab176490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 47,65 kDa.
ELISA Use at an assay dependent concentration.

(Cellular and membrane)

Target

  • Function
    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • Post-translational
    modifications
    Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all

Images

  • Anti-beta 2 Adrenergic Receptor antibody (ab176490) at 2.5 µg/ml + Rat heart membrane extract at 20 µg

    Predicted band size: 47,65 kDa
    Additional bands at: 65 kDa (possible post-translational modification)

  • Cellular ELISA: HEK293 cells expressing beta 2 Adrenergic Receptor were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CPG12177) and probed with ab176490 (1/1000 of a stock solution of 1µg/μL ) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).

  • Membrane ELISA: Lewis rat brain membranes (5 µg/well) were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CGP12177) and probed with ab176490 (1/1000 of a stock solution of 1µg/μL) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).

References

ab176490 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab176490.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up