Product nameAnti-beta 2 Adrenergic Receptor antibody
See all beta 2 Adrenergic Receptor primary antibodies
DescriptionRabbit polyclonal to beta 2 Adrenergic Receptor
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Horse, Guinea pig, Hamster, Cat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Mouse beta 2 Adrenergic Receptor (N terminal). The exact sequence is proprietary.
Database link: P18762
- Rat heart membrane extract, Rat brain stem, HEK293 cells expressing beta 2 Adrenergic Receptor, Lewis rat brain membranes
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 47,65 kDa.|
|ELISA||Use at an assay dependent concentration.
(Cellular and membrane)
FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
modificationsPalmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
Cellular localizationCell membrane.
- Information by UniProt
- ADRB2 antibody
- ADRB2_HUMAN antibody
- ADRB2R antibody
Anti-beta 2 Adrenergic Receptor antibody (ab176490) at 2.5 µg/ml + Rat heart membrane extract at 20 µg
Predicted band size: 47,65 kDa
Additional bands at: 65 kDa (possible post-translational modification)
Cellular ELISA: HEK293 cells expressing beta 2 Adrenergic Receptor were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CPG12177) and probed with ab176490 (1/1000 of a stock solution of 1µg/μL ) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
Membrane ELISA: Lewis rat brain membranes (5 µg/well) were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CGP12177) and probed with ab176490 (1/1000 of a stock solution of 1µg/μL) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
ab176490 has not yet been referenced specifically in any publications.