Anti-beta 2 Adrenergic Receptor antibody (ab61778)


  • Product name
    Anti-beta 2 Adrenergic Receptor antibody
    See all beta 2 Adrenergic Receptor primary antibodies
  • Description
    Rabbit polyclonal to beta 2 Adrenergic Receptor
  • Host species
  • Specificity
    ab61778 detects endogenous levels of total beta 2 Adrenergic Receptor protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human beta 2 Adrenergic Receptor around the phosphorylation site of serine 346.

  • Positive control
    • Human brain tissue. HeLa cells.
  • General notes

    Based on immunogen sequence homology data ab61778 is predicted to react with mouse species however customers has provided us mixed feedback which is why we no longer support its cross reactivity with mouse samples.  



Our Abpromise guarantee covers the use of ab61778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 46 kDa. (see Abreview)
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
Flow Cyt 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • Function
    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • Post-translational
    Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all


  • ab61778, at 1/50 - 1/100 dilution, staining beta 2 Adrenergic Receptor in paraffin-embedded human brain tissue by immunohistochemistry, in the absence (left panel) or presence (right panel) of the immunising peptide.
  • ab61778, at 1/500 - 1/1000 dilution, staining beta 2 Adrenergic Receptor in HeLa cells by immunofluorescence, in the absence (left panel) or presence (right panel) of the immunising peptide.
  • ab61778 staining the beta 2 adrenergic receptor in Human peripheral blood monomuclear cells by Flow Cytometry. Cells was prepared by Ficoll gradient separation in PBS/BSA and was fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS + 0.5% BSA) for 1 hour at 18°C. Ab7007 (1/100) was used as secondary antibody.
    Gating Strategy: Monocytes

    See Abreview

  • All lanes : Anti-beta 2 Adrenergic Receptor antibody (ab61778)

    Lane 1 : NIH-3T3 cell lysate.
    Lane 2 : NIH-3T3 cell lysate with blocking peptide.

    Predicted band size: 46 kDa
    Observed band size: 48 kDa (why is the actual band size different from the predicted?)

  • abb61778 staining of the β2-adrenergic receptor in mesenchymal stem cells by flow cytometry. Histogram of adrenergic receptors fluorescence shown as the red curve, IgG control shown as the grey curve.


This product has been referenced in:
  • Lo CY  et al. Reduced suppressive effect of ß2-adrenoceptor agonist on fibrocyte function in severe asthma. Respir Res 18:194 (2017). Read more (PubMed: 29162108) »
  • Tyurin-Kuzmin PA  et al. Activation of ß-adrenergic receptors is required for elevated a1A-adrenoreceptors expression and signaling in mesenchymal stromal cells. Sci Rep 6:32835 (2016). Flow Cyt . Read more (PubMed: 27596381) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Flow Cytometry
Mouse Cell (Spleen cells)
Spleen cells

Abcam user community

Verified customer

Submitted May 22 2017

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

We would be pleased to arrange a replacement product free of charge. I am forwarding these details on to our Distributor’s ...

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Thank you for sending us more information about the protocol used. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital informatio...

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Thank you for contacting us.

Regarding ab61778, I have checked our database and have found that this antibody has been validated in many applications including Western blot and so far, we have no reason to believe that it is not specific. Read More

Thank you for your reply.

The immunogen for ab61778 is a synthetic non-phosphopeptide derived from human beta 2 Adrenergic Receptor around the phosphorylation site of serine 346. Therefore based on the information available on http://www.unip...

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Thank you for contacting Abcam.

The only information we have on ab61778 staining patterns in IHC-P are in the attached file. As I am not an expert in the staining pattern of this antibody, I thought it would be more useful for you to be able ...

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Thank you for contacting us. Unfortunately the peptides used as the immunogens for these products are not available in our catalog.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advic...

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Thank you for participating in our recent customer satisfaction survey.

I am happy to hear that the replacement of ab36956 worked well! We appreciate your feedback and the time you took in your experiments.

If you need any other pr...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for contacting us. I hope that the suggestions which were made help you to resolve the issues which you have been experiencing. As requested, I have arranged for you to receive positive control lysates ab71719 and ab29330 free of charge. T...

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