Overview

  • Product name

    Anti-beta 2 Adrenergic Receptor antibody - C-terminal
    See all beta 2 Adrenergic Receptor primary antibodies
  • Description

    Rabbit polyclonal to beta 2 Adrenergic Receptor - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Monkey
  • Immunogen

    Synthetic peptide corresponding to Human beta 2 Adrenergic Receptor (C terminal). Carrier-protein conjugated synthetic peptide encompassing a sequence within aa 340-413. The exact sequence is proprietary.
    Database link: P07550

  • Positive control

    • WB: A431, HepG2, PC-12 whole cell extracts. IHC-P: Human colon carcinoma. HeLa xenograft tissue. ICC/IF: This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC3.

Properties

Applications

Our Abpromise guarantee covers the use of ab137494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • Post-translational
    modifications

    Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all

Images

  • All lanes : Anti-beta 2 Adrenergic Receptor antibody - C-terminal (ab137494) at 1/500 dilution

    Lane 1 : A431 whole cell extracts
    Lane 2 : HepG2 whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 46 kDa



    10% gel. Running: 80V, 15min; 140V, 40 min. Transfer: Semi-dry, 18 V, 60 min (Nitrocellulose membrane). Blocking: 5% non-fat milk in TBST, RT, 60 min. Primary antibody incubation: 4°C overnight. Washing: 5 ml TBST, 4 x 5 min. Exposure using Western blot HRP substrate. 

     

     

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma labeling beta 2 Adrenergic Receptor with ab137494 at 1/250 dilution. Cytosol staining is observed. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

  • Anti-beta 2 Adrenergic Receptor antibody - C-terminal (ab137494) at 1/500 dilution + PC12 whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 46 kDa



    10% SDS PAGE
  • ab137494 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab137494 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin-embedded Hela xenograft, labelling beta 2 Adrenergic Receptor with ab137494 at 1/500 dilution.

References

This product has been referenced in:

  • Sun F  et al. PKA/ß2-AR-Gs/Gi signaling pathway is associated with anti-inflammatory and pro-apoptotic effects of Fuzi and Banxia combination on rats subjected to pressure overload. J Ethnopharmacol 235:375-384 (2019). Read more (PubMed: 30738114) »
  • Sun F  et al. Stage-dependent changes of ß2-adrenergic receptor signaling in right ventricular remodeling in monocrotaline-induced pulmonary arterial hypertension. Int J Mol Med 41:2493-2504 (2018). Read more (PubMed: 29393391) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Rat Cell (Blood leukocytes)
Permeabilization
No
Specification
Blood leukocytes
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 26 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up