Key features and details
- Rabbit polyclonal to beta 2 Adrenergic Receptor (phospho S346)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-beta 2 Adrenergic Receptor (phospho S346) antibody
See all beta 2 Adrenergic Receptor primary antibodies
DescriptionRabbit polyclonal to beta 2 Adrenergic Receptor (phospho S346)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human beta 2 Adrenergic Receptor (phospho S346) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of serine 346 (R-S-S(p)-L-K). NP_000015.1
Database link: P07550
- HepG2 cell extracts treated with Nocodazole.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192821 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab192821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 46 kDa.|
FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
modificationsPalmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
Cellular localizationCell membrane.
- Information by UniProt
- ADRB2 antibody
- ADRB2_HUMAN antibody
- ADRB2R antibody
All lanes : Anti-beta 2 Adrenergic Receptor (phospho S346) antibody (ab192821) at 1/500 dilution
Lane 1 : HepG2 cell extracts treated with Nocodazole - no peptide
Lane 2 : HepG2 cell extracts treated with Nocodazole.- with the antigen-specific peptide
Predicted band size: 46 kDa
HepG2 cell extracts treated with Nocodazole
ab192821 has not yet been referenced specifically in any publications.