Overview

  • Product name

    Anti-beta 2 Adrenergic Receptor (phospho S346) antibody
    See all beta 2 Adrenergic Receptor primary antibodies
  • Description

    Rabbit polyclonal to beta 2 Adrenergic Receptor (phospho S346)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human beta 2 Adrenergic Receptor (phospho S346) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of serine 346 (R-S-S(p)-L-K). NP_000015.1
    Database link: P07550

  • Positive control

    • HepG2 cell extracts treated with Nocodazole.

Properties

Applications

Our Abpromise guarantee covers the use of ab192821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.

Target

  • Function

    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • Post-translational
    modifications

    Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all

Images

  • All lanes : Anti-beta 2 Adrenergic Receptor (phospho S346) antibody (ab192821) at 1/500 dilution

    Lane 1 : HepG2 cell extracts treated with Nocodazole - no peptide
    Lane 2 : HepG2 cell extracts treated with Nocodazole.- with the antigen-specific peptide

    Predicted band size: 46 kDa



    HepG2 cell extracts treated with Nocodazole

References

ab192821 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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