Overview

  • Product name
    Anti-beta 2 Defensin / hBD-2 antibody
    See all beta 2 Defensin / hBD-2 primary antibodies
  • Description
    Goat polyclonal to beta 2 Defensin / hBD-2
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, Sandwich ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human beta 2 Defensin/ hBD-2 aa 24-64. Highly pure (>98%) recombinant Human beta 2 Defensin/ hBD-2. Full length mature beta 2 Defensin/ hBD-2 used as immunogen.
    Sequence:

    GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P


    Database link: O15263

  • Positive control
    • recombinant beta 2 Defensin / hBD-2 ab9872
  • General notes

    hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.

    Previously labelled as beta 2 Defensin.

Properties

  • Form
    Lyophilised:Centrifuge vial prior to opening. Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described, then no further reconstitution is necessary. The lyophilized antibody is stable for at least 2 years from date of receipt at -20C. The reconstituted antibody is stable for at least two weeks at 2-8C. Frozen aliquots are stable for at least 6 months when stored at -20C. Avoid repeated freeze-thaw cycles.
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.

To detect beta 2 Defensin / hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant beta 2 Defensin / hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions

Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.

To detect beta 2 Defensin / hBD-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83509 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant beta 2 Defensin / hBD-2.

Neutralising Use a concentration of 5 - 8 µg/ml.

To yield one-half maximal inhibition [ND50] of the biological activity of Human beta 2 Defensin / hBD-2 (1.0 μg/ml), a concentration of 5.0 - 8.0 μg/ml of this antibody is required.

Target

  • Function
    Has antibacterial activity.
  • Tissue specificity
    Expressed in the skin and respiratory tract.
  • Sequence similarities
    Belongs to the beta-defensin family. LAP/TAP subfamily.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BD 2 antibody
    • BD-2 antibody
    • BD2 antibody
    • beta 2 antibody
    • beta 2 Defensin antibody
    • Beta defensin 2 antibody
    • Beta defensin 4B antibody
    • Beta-defensin 2 antibody
    • Beta-defensin 4A antibody
    • DEF B2 antibody
    • DEF B4 antibody
    • DEFB 102 antibody
    • DEFB 2 antibody
    • DEFB 4 antibody
    • DEFB102 antibody
    • DEFB2 antibody
    • DEFB2, formerly antibody
    • DEFB4 antibody
    • DEFB4, formerly antibody
    • DEFB4B antibody
    • DEFB4P antibody
    • Defensin antibody
    • Defensin beta 2 antibody
    • Defensin, beta 4 antibody
    • Defensin, beta 4, pseudogene antibody
    • Defensin, beta 4A antibody
    • Defensin, beta 4B antibody
    • Defensin, beta, 2, formerly antibody
    • Defensin, beta, 4, formerly antibody
    • DFB4A_HUMAN antibody
    • HBD 2 antibody
    • hBD-2 antibody
    • HBD2 antibody
    • mBD-2 antibody
    • SAP 1 antibody
    • SAP1 antibody
    • Skin antimicrobial peptide 1 antibody
    • Skin-antimicrobial peptide 1 antibody
    see all

References

This product has been referenced in:
  • Landman S  et al. Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model. Sci Rep 8:10044 (2018). Read more (PubMed: 29968819) »
  • de Koning HD  et al. A comprehensive analysis of pattern recognition receptors in normal and inflamed human epidermis: upregulation of dectin-1 in psoriasis. J Invest Dermatol 130:2611-20 (2010). Read more (PubMed: 20631729) »
See all 5 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Answer

The exact immunogen sequence for these antibodies is proprietary so I am sorry we are unable to disclose this information. The immunogen however corresponds to the mature form of Beta 2 defensin protein. We do have sequence listed for ab14426 and ab66072. I wonder if these can help.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (supernatens from keratinocytes)
Specification
supernatens from keratinocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Direct

Abcam user community

Verified customer

Submitted Jul 18 2013

Question
Answer

Thank you for your interest in Abcam.

We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies directly. However, we are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services, please visit:

http://www.epitomics.com/services/

I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

Read More

Answer

Thank you for submitting the Abreviews with ab9871, ab15117, ab110534 and ab18499 using dot blot. The results look really good.

I will be submitting your reviews manually. In order to issue the Abpoints generated to the relevant account, could you please confirm that you want them sent to your account (xxxxx).

I have issued instructions that the discount codes issued can be activated prior to the publishing of the reviews so you can now go ahead and use them to claim your free primary antibody.

Again thank you for providing us with these reviews. If you have any further queries please do not hesitate to contact us again.

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Question
Answer

Thank you for contacting us.

As discussed we have many anti goat antibodies that can be used in ELISA and ICC/IF.

Idealy enzyme conjugated antibodies e.g. HRP, Alkaline phosphatase are the best suitable antibodies in ELISA. However these will not work in Immunofluorescence unless further steps in protocol applied.

We have FITC, TRITC or Cy5 conjugated antibodies in catalogue that can work in Immunofluorescence and Fluorescence ELISA.

Another option is choosing Biotin conjugated secondary antibody and then buying strepavidin or streptavidin conjugated HRP (ab7403).

ab5753, ab6740, ab6884, ab7124, ab7131 coul dbe a good option.

I know it sounds complicated however this is only way to combine ELISA and ICC/IF with one secondary antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (keratinocytes)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Loading amount
500000 cells
Treatment
see below (Additional notes)
Specification
keratinocytes
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 27 2012

Answer

DISCOUNT CODE: xxxxx Expiration date: 31/03/2012 I am very pleased to hear your customer would like to test ab9871 in Dot Blot. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for Dot Blot and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your customers Abreview. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount

Read More

Answer

Thank you for contacting us. We would be very interested in your customer testing ab9871 with Dot Blot with human samples. If you could please send me the name, email address and institute of your customer I will create the discount code for you.

Read More

Answer

Thank you for your response and your interest. We look forward to receiving your feedback on ab9871 and publishing the Abreview. We are unable to issue discount codes for testing in retrospect for those antibodies which were purchased in the past. Customers need to ask for permission for testing and agree with the conditions. Please could you read the "Terms and Conditions" at this site to understand our policy: www.abcam.com/collaborationdiscount Follow these easy steps for a 100% testing discount: Email technical@abcam.com or us.technical@abcam.com and tell our Scientific Support team the product, application, and species you are interested in testing Our scientists will email you a personal discount code to give you a 100% discount on your next product Purchase the product at regular price Test the product in your species and application Submit your results: www.abcam.com/abreviews Once the Abreview is submitted, the discount code will become active Place a new order by phone, fax, or web and include your 100% testing discount code If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

This antibody is produced using Recombinant Human beta-defensin-2 (BD-2) as the immunizing antigen. BD-2 is an antimicrobial peptide which belongs to the distinct family of beta-defensins. Recent studies have shown that the beta-defensins are present in the human airways and may be relevant to the pathogenesis of cystic fibrosis lung disease. Recombinant Human BD-2 is a 4.3 kDa protein containing 41 amino acid residues. BD-2 sequence GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P Please see the ELISA protocol linked on the right hand side of the Abcam homepage.

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1-10 of 13 Abreviews or Q&A

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