• Product name
    Anti-beta 2 Defensin antibody
    See all beta 2 Defensin primary antibodies
  • Description
    Goat polyclonal to beta 2 Defensin
  • Host species
  • Tested applications
    Suitable for: WB, Sandwich ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human beta 2 Defensin aa 24-64. Highly pure (>98%) recombinant Human beta 2 Defensin. Full length mature Beta-defensin 2 used as immunogen.


    Database link: O15263

  • Positive control
    • recombinant hBD-2 (human Beta Defensin-2) ab9872
  • General notes

    hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.


  • Form
    Lyophilised:Centrifuge vial prior to opening. Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described, then no further reconstitution is necessary. The lyophilized antibody is stable for at least 2 years from date of receipt at -20C. The reconstituted antibody is stable for at least two weeks at 2-8C. Frozen aliquots are stable for at least 6 months when stored at -20C. Avoid repeated freeze-thaw cycles.
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab9871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.

To detect beta 2 Defensin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant beta 2 Defensin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions

Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.

To detect beta 2 Defensin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83509 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant beta 2 Defensin.

Neutralising Use a concentration of 5 - 8 µg/ml.

To yield one-half maximal inhibition [ND50] of the biological activity of Human Beta 2 Defensin (1.0 μg/ml), a concentration of 5.0 - 8.0 μg/ml of this antibody is required.


  • Function
    Has antibacterial activity.
  • Tissue specificity
    Expressed in the skin and respiratory tract.
  • Sequence similarities
    Belongs to the beta-defensin family. LAP/TAP subfamily.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BD 2 antibody
    • BD-2 antibody
    • BD2 antibody
    • beta 2 antibody
    • Beta defensin 2 antibody
    • Beta defensin 4B antibody
    • Beta-defensin 2 antibody
    • Beta-defensin 4A antibody
    • DEF B2 antibody
    • DEF B4 antibody
    • DEFB 102 antibody
    • DEFB 2 antibody
    • DEFB 4 antibody
    • DEFB102 antibody
    • DEFB2 antibody
    • DEFB2, formerly antibody
    • DEFB4 antibody
    • DEFB4, formerly antibody
    • DEFB4B antibody
    • DEFB4P antibody
    • Defensin antibody
    • Defensin beta 2 antibody
    • Defensin, beta 4 antibody
    • Defensin, beta 4, pseudogene antibody
    • Defensin, beta 4A antibody
    • Defensin, beta 4B antibody
    • Defensin, beta, 2, formerly antibody
    • Defensin, beta, 4, formerly antibody
    • DFB4A_HUMAN antibody
    • HBD 2 antibody
    • hBD-2 antibody
    • HBD2 antibody
    • mBD-2 antibody
    • SAP 1 antibody
    • SAP1 antibody
    • Skin antimicrobial peptide 1 antibody
    • Skin-antimicrobial peptide 1 antibody
    see all


This product has been referenced in:
  • de Koning HD  et al. A comprehensive analysis of pattern recognition receptors in normal and inflamed human epidermis: upregulation of dectin-1 in psoriasis. J Invest Dermatol 130:2611-20 (2010). Read more (PubMed: 20631729) »
  • Pignatti P  et al. Tracheostomy and related host-pathogen interaction are associated with airway inflammation as characterized by tracheal aspirate analysis. Respir Med 103:201-8 (2009). Read more (PubMed: 18980836) »

See all 4 Publications for this product

Customer reviews and Q&As

The exact immunogen sequence for these antibodies is proprietary so I am sorry we are unable to disclose this information. The immunogen however corresponds to the mature form of Beta 2 defensin protein. We do have sequence listed for ab14426 and ab66...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell (supernatens from keratinocytes)
supernatens from keratinocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 17 2013

Thank you for your interest in Abcam.

We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies directly. However, we are happy to be able to offer custom antibody services...

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Thank you for submitting the Abreviews with ab9871, ab15117, ab110534 and ab18499 using dot blot. The results look really good.

I will be submitting your reviews manually. In order to issue the Abpoints generated to the relevant account, cou...

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Thank you for contacting us.

As discussed we have many anti goat antibodies that can be used in ELISA and ICC/IF.

Idealy enzyme conjugated antibodies e.g. HRP, Alkaline phosphatase are the best suitable antibodies in ELISA. However...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (keratinocytes)
Loading amount
500000 cells
see below (Additional notes)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 27 2012

DISCOUNT CODE: xxxxx Expiration date: 31/03/2012 I am very pleased to hear your customer would like to test ab9871 in Dot Blot. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abr...

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Thank you for contacting us. We would be very interested in your customer testing ab9871 with Dot Blot with human samples. If you could please send me the name, email address and institute of your customer I will create the discount code for you.

Thank you for your response and your interest. We look forward to receiving your feedback on ab9871 and publishing the Abreview. We are unable to issue discount codes for testing in retrospect for those antibodies which were purchased in the past. ...

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This antibody is produced using Recombinant Human beta-defensin-2 (BD-2) as the immunizing antigen. BD-2 is an antimicrobial peptide which belongs to the distinct family of beta-defensins. Recent studies have shown that the beta-defensins are present i...

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1-10 of 13 Abreviews or Q&A


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