Anti-beta 2 Defensin/BD-2 antibody (ab9871)
Key features and details
- Goat polyclonal to beta 2 Defensin/BD-2
- Suitable for: Sandwich ELISA, Neutralising, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta 2 Defensin/BD-2 antibody
See all beta 2 Defensin/BD-2 primary antibodies -
Description
Goat polyclonal to beta 2 Defensin/BD-2 -
Host species
Goat -
Tested applications
Suitable for: Sandwich ELISA, Neutralising, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human beta 2 Defensin/BD-2 aa 1 to the C-terminus. Highly pure (>98%) recombinant Human beta 2 Defensin/hBD-2. Full length mature beta 2 Defensin hBD-2 used as immunogen.
Database link: O15263 -
Positive control
- recombinant beta 2 Defensin/BD-2 ab9872
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General notes
hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.
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Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described, then no further reconstitution is necessary. The lyophilized antibody is stable for at least 2 years from date of receipt at -20C. The reconstituted antibody is stable for at least two weeks at 2-8C. Frozen aliquots are stable for at least 6 months when stored at -20C. Avoid repeated freeze-thaw cycles. -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration.
To detect beta 2 Defensin/hBD-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83509 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant beta 2 Defensin/hBD-2. |
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Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of Human beta 2 Defensin/hBD-2 (1.0 ug/ml), a concentration of 5.0 - 8.0 ug/ml of this antibody is required. |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 7 kDa.
To detect beta 2 Defensin/hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant beta 2 Defensin/hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions |
Notes |
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Sandwich ELISA
Use at an assay dependent concentration. To detect beta 2 Defensin/hBD-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83509 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant beta 2 Defensin/hBD-2. |
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of Human beta 2 Defensin/hBD-2 (1.0 ug/ml), a concentration of 5.0 - 8.0 ug/ml of this antibody is required. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 7 kDa. To detect beta 2 Defensin/hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant beta 2 Defensin/hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions |
Target
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Function
Has antibacterial activity. -
Tissue specificity
Expressed in the skin and respiratory tract. -
Sequence similarities
Belongs to the beta-defensin family. LAP/TAP subfamily. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 100289462 Human
- Entrez Gene: 1673 Human
- Omim: 602215 Human
- SwissProt: O15263 Human
- Unigene: 105924 Human
- Unigene: 721744 Human
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Alternative names
- BD 2 antibody
- BD-2 antibody
- BD2 antibody
see all
Datasheets and documents
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Datasheet download
References (8)
ab9871 has been referenced in 8 publications.
- Johnson V et al. Activated Mesenchymal Stromal Cell Therapy for Treatment of Multi-Drug Resistant Bacterial Infections in Dogs. Front Vet Sci 9:925701 (2022). PubMed: 35812842
- Chow L et al. Antibacterial activity of human mesenchymal stem cells mediated directly by constitutively secreted factors and indirectly by activation of innate immune effector cells. Stem Cells Transl Med 9:235-249 (2020). PubMed: 31702119
- Smits JPH et al. Targeting the Cutaneous Microbiota in Atopic Dermatitis by Coal Tar via AHR-Dependent Induction of Antimicrobial Peptides. J Invest Dermatol 140:415-424.e10 (2020). PubMed: 31344386
- Landman S et al. Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model. Sci Rep 8:10044 (2018). PubMed: 29968819
- de Koning HD et al. A comprehensive analysis of pattern recognition receptors in normal and inflamed human epidermis: upregulation of dectin-1 in psoriasis. J Invest Dermatol 130:2611-20 (2010). PubMed: 20631729
- Pignatti P et al. Tracheostomy and related host-pathogen interaction are associated with airway inflammation as characterized by tracheal aspirate analysis. Respir Med 103:201-8 (2009). PubMed: 18980836
- Herr C et al. Suppression of pulmonary innate host defence in smokers. Thorax 64:144-9 (2009). PubMed: 18852155
- Fahlgren A et al. Increased expression of antimicrobial peptides and lysozyme in colonic epithelial cells of patients with ulcerative colitis. Clin Exp Immunol 131:90-101 (2003). PubMed: 12519391