Key features and details
- Rabbit polyclonal to beta 2 Defensin/BD-2
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG
Product nameAnti-beta 2 Defensin/BD-2 antibody
See all beta 2 Defensin/BD-2 primary antibodies
DescriptionRabbit polyclonal to beta 2 Defensin/BD-2
Human 7 kDa beta 2 Defensin / hBD-2.
Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to beta 2 Defensin/BD-2.
Previously labelled as beta 2 Defensin.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: None
Concentration information loading...
Our Abpromise guarantee covers the use of ab63982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 7 kDa.|
|IHC-P||1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionHas antibacterial activity.
Tissue specificityExpressed in the skin and respiratory tract.
Sequence similaritiesBelongs to the beta-defensin family. LAP/TAP subfamily.
- Information by UniProt
- BD 2 antibody
- BD-2 antibody
- BD2 antibody
ab63982 (1/500) staining beta 2 Defensin/BD-2 in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of a small population of cells within the tonsillar tissue.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ab63982 staining beta 2 Defensin/BD-2 in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/500 dilution for 1 hour at 25°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
ab63982 has been referenced in 11 publications.
- Peled A et al. Loss-of-function mutations in caspase recruitment domain-containing protein 14 (CARD14) are associated with a severe variant of atopic dermatitis. J Allergy Clin Immunol 143:173-181.e10 (2019). PubMed: 30248356
- Dagnelie MA et al. Assessment of seven protocols to prepare Cutibacterium acnes bacterial lysates to measure its immunogenic potential and review of the literature. Anaerobe 57:75-81 (2019). PubMed: 30935994
- Guo J et al. TLR4 Agonist Monophosphoryl Lipid A Alleviated Radiation-Induced Intestinal Injury. J Immunol Res 2019:2121095 (2019). PubMed: 31275998
- Patra V et al. Unique profile of antimicrobial peptide expression in polymorphic light eruption lesions compared to healthy skin, atopic dermatitis, and psoriasis. Photodermatol Photoimmunol Photomed 34:137-144 (2018). IHC-P ; Human . PubMed: 29044786
- Ali ASM et al. Targeting Deficiencies in the TLR5 Mediated Vaginal Response to Treat Female Recurrent Urinary Tract Infection. Sci Rep 7:11039 (2017). PubMed: 28887442