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Cardiovascular Blood Serum Proteins
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Anti-beta 2 Microglobulin antibody (ab175031)

  • Datasheet
  • SDS
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Western blot - Anti-beta 2 Microglobulin antibody (ab175031)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody (ab175031)

Key features and details

  • Rabbit polyclonal to beta 2 Microglobulin
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-beta 2 Microglobulin antibody
    See all beta 2 Microglobulin primary antibodies
  • Description

    Rabbit polyclonal to beta 2 Microglobulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human beta 2 Microglobulin.
    Database link: P61769

  • Positive control

    • Jurkat, COS-7, HeLa and K562 cell extracts. Mouse lung, liver and spleen extracts.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cancer
    • Tumor biomarkers
    • Globulins
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • WBC Antigens
    • HLA

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Mouse spleen tissue lysate - total protein (ab29293)
    • Mouse lung normal tissue lysate - total protein (ab29297)
    • Mouse liver tissue lysate - total protein (ab29301)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
    • K562 whole cell lysate (ab7911)
    • Mouse spleen tissue lysate - total protein (ab7937)
  • Recombinant Protein

    • Recombinant Human beta 2 Microglobulin protein (ab98116)

Applications

Our Abpromise guarantee covers the use of ab175031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 14 kDa.

Target

  • Function

    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Involvement in disease

    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • Sequence similarities

    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Cellular localization

    Secreted. Detected in serum and urine.
  • Target information above from: UniProt accession P61769 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 567 Human
    • Entrez Gene: 12010 Mouse
    • Entrez Gene: 24223 Rat
    • Omim: 109700 Human
    • SwissProt: P61769 Human
    • SwissProt: P01887 Mouse
    • SwissProt: P07151 Rat
    • Unigene: 534255 Human
    • Unigene: 702947 Human
    • Unigene: 163 Mouse
    • Unigene: 1868 Rat
    see all
  • Alternative names

    • B2M antibody
    • B2MG_HUMAN antibody
    • Beta 2 microglobin antibody
    • Beta 2 microglobulin antibody
    • Beta 2 microglobulin precursor antibody
    • Beta chain of mhc class 1 proteins antibody
    • Beta chain of MHC class I molecules antibody
    • Beta-2-microglobulin form pI 5.3 antibody
    • CDABP0092 antibody
    • Hdcma22p antibody
    • IMD43 antibody
    see all

Images

  • Western blot - Anti-beta 2 Microglobulin antibody (ab175031)
    Western blot - Anti-beta 2 Microglobulin antibody (ab175031)
    All lanes : Anti-beta 2 Microglobulin antibody (ab175031) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : COS-7 cell extract
    Lane 3 : HeLa cell extract
    Lane 4 : K562 cell extract
    Lane 5 : Mouse lung extract
    Lane 6 : Mouse liver extract
    Lane 7 : Mouse spleen extract

    Predicted band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody (ab175031)
    Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody (ab175031)
    Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab175031. Blue DAPI for nuclear staining.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (3)

    Publishing research using ab175031? Please let us know so that we can cite the reference in this datasheet.

    ab175031 has been referenced in 3 publications.

    • Fels J  et al. Cadmium Complexed with ß2-Microglubulin, Albumin and Lipocalin-2 rather than Metallothionein Cause Megalin:Cubilin Dependent Toxicity of the Renal Proximal Tubule. Int J Mol Sci 20:N/A (2019). PubMed: 31091675
    • Basu A  et al. A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells associated with Enhanced Wound Healing. Sci Rep 8:6205 (2018). PubMed: 29670130
    • Wang H  et al. HGF Gene Modification in Mesenchymal Stem Cells Reduces Radiation-Induced Intestinal Injury by Modulating Immunity. PLoS One 10:e0124420 (2015). IHC . PubMed: 25933295

    Customer reviews and Q&As

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