Overview

  • Product name
    Anti-beta 2 Microglobulin antibody
    See all beta 2 Microglobulin primary antibodies
  • Description
    Rabbit polyclonal to beta 2 Microglobulin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human beta 2 Microglobulin.
    Database link: P61769

  • Positive control
    • Jurkat, COS-7, HeLa and K562 cell extracts. Mouse lung, liver and spleen extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab175031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/2000. Predicted molecular weight: 14 kDa.

Target

  • Function
    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Involvement in disease
    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • Sequence similarities
    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Cellular localization
    Secreted. Detected in serum and urine.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2M antibody
    • B2MG_HUMAN antibody
    • Beta 2 microglobin antibody
    • Beta 2 microglobulin antibody
    • Beta 2 microglobulin precursor antibody
    • Beta chain of mhc class 1 proteins antibody
    • Beta chain of MHC class I molecules antibody
    • Beta-2-microglobulin form pI 5.3 antibody
    • CDABP0092 antibody
    • Hdcma22p antibody
    • IMD43 antibody
    see all

Images

  • All lanes : Anti-beta 2 Microglobulin antibody (ab175031) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : COS-7 cell extract
    Lane 3 : HeLa cell extract
    Lane 4 : K562 cell extract
    Lane 5 : Mouse lung extract
    Lane 6 : Mouse liver extract
    Lane 7 : Mouse spleen extract

    Predicted band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse heart tissue labelling beta 2 Microglobulin with ab175031 at 1/200. Magnification: 200x.

  • Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab175031. Blue DAPI for nuclear staining.

References

This product has been referenced in:
  • Basu A  et al. A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells associated with Enhanced Wound Healing. Sci Rep 8:6205 (2018). Read more (PubMed: 29670130) »
  • Wang H  et al. HGF Gene Modification in Mesenchymal Stem Cells Reduces Radiation-Induced Intestinal Injury by Modulating Immunity. PLoS One 10:e0124420 (2015). IHC . Read more (PubMed: 25933295) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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