Key features and details
- Goat polyclonal to beta 2 Microglobulin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-beta 2 Microglobulin antibody
See all beta 2 Microglobulin primary antibodies
DescriptionGoat polyclonal to beta 2 Microglobulin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
- WB: Human duodenum lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223495 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 14 kDa).
1 hour primary incubation is recommended for this product.
FunctionComponent of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Involvement in diseaseDefects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
Sequence similaritiesBelongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients.
Cellular localizationSecreted. Detected in serum and urine.
- Information by UniProt
- B2M antibody
- B2MG_HUMAN antibody
- Beta 2 microglobin antibody
Anti-beta 2 Microglobulin antibody (ab223495) at 0.03 µg/ml + Human duodenum lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 14 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab223495 has not yet been referenced specifically in any publications.