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Thank you for your replacement AB6608 which our customer has received in the meantime. Unfortunately the problem (many spots on the blot) does also appear with this new vial (which is of the same lot 7396)! So the question is, what is the cause for this problem? Do you have any more suggestions or feedback from your lab/QC? Kind regards,
Asked on Jul 09 2004
Thank you for your patience. Actually we've never seen a blot like this before. We consulted with a couple of senior staffs in our company. We came out several suggestions as following: 1. Filter the whatever blocking reagent they use, may add 10% Goat serum to the blocking buffer, do the blocking for at least 1 h, R.T. 2. Do a 1st antibody negative control blot side-by-side with a complete WB 2. Since the specific bands are so strong, further dilute the antibody. The primary antibody incubation time can be shortened to 1h, R.T. There are many factors beyond our control. Please let us know if these could help. Thank you.
Answered on Jul 14 2004