Overview

  • Product name
    Anti-beta 2 Microglobulin antibody [B2M-01]
    See all beta 2 Microglobulin primary antibodies
  • Description
    Mouse monoclonal [B2M-01] to beta 2 Microglobulin
  • Host species
    Mouse
  • Specificity
    This antibody reacts with beta-2-microglobulin, associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble forms of beta-2-microglobulin. The antibody detected beta-2-microglobulin in U-937 and RAJI cell lines in WB in non reducing conditions however failed to detect a band in JEG-3 and A431 cell lysates. All lysates gave a signal in direct ELISA.
  • Tested applications
    Suitable for: ELISA, Flow Cyt, IP, RIA, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rabbit, Chicken, Cow, Dog, Pig
  • Immunogen

    Full length native protein (purified) (Human).

  • Positive control
    • U-937 and RAJI cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
RIA Use at an assay dependent concentration. Dissociation constant of the antibody-soluble b2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.
WB Use a concentration of 2 µg/ml. Use under non reducing condition. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.

Target

  • Function
    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Involvement in disease
    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • Sequence similarities
    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Cellular localization
    Secreted. Detected in serum and urine.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2M antibody
    • B2MG_HUMAN antibody
    • Beta 2 microglobin antibody
    • Beta 2 microglobulin antibody
    • Beta 2 microglobulin precursor antibody
    • Beta chain of mhc class 1 proteins antibody
    • Beta chain of MHC class I molecules antibody
    • Beta-2-microglobulin form pI 5.3 antibody
    • CDABP0092 antibody
    • Hdcma22p antibody
    • IMD43 antibody
    see all

Images

  • All lanes : Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) at 2 µg/ml

    Lane 1 : JEG-3 cell lysate
    Lane 2 : A-431 cell lysate
    Lane 3 : U-937 cell lysate
    Lane 4 : Raji cell lysate

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 13 kDa



    Lysates were separated on 15% SDS–PAGE gel and electrotransferred to a PVDF membrane. The membrane was blocked in PBS/Tween buffer containing 5% non fat dry milk for 2 hours and probed with ab759 at 4°C overnight.

References

This product has been referenced in:
  • Rodríguez-Rodríguez M  et al. Activation of Peptidylarginine Deiminase in the Salivary Glands ofBalb/cMice Drives the Citrullination of Ro and La Ribonucleoproteins. J Immunol Res 2017:8959687 (2017). Read more (PubMed: 29318161) »
  • Raha-Chowdhury R  et al. Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci 16:24 (2015). WB . Read more (PubMed: 25896789) »
See all 11 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.1M PBS with 3% TritonX100
Fixative
Paraformaldehyde

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 14 2014

Answer

Thank you for contacting us for technical support regarding ab759. I have contacted the laboratory and was able to find out that indeed non reducing conditions are advised with this antibody. I have added immediately on our datasheet (accessible below) this information and the image of the blot where four lysates were tested and where you can see a clear band of beta 2 Microglobulin with two lysates (U-937 cell lysate and Raji cell lysate). We expected a signal on Jeg3 cells since on the native lysate it worked by ELISA (ab759 as detecting antibody, directly conjugated with HRP). However, for WB condition, the lysate was not reduced (only denatured by SDS), which may explain the lack of signal with this lysate (furthermore each lysate preparation was not done at the same time). Therefore collectively, the antibody works in western blotting in non reduced conditions and you may find you can achieve a signal with non reducing conditions. May I also suggest to use a 15 or 20% gel to achieve a good separation and run a positive control of U-937 cell lysate or Raji cell lysate? We used a concentration of 2ug/ml of ab729, incubating overnight and blocking for 2hrs with 5% non fat milk. I hope this information will help you, please do not hesitate to contact me again if I can be of further assistance,

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Question
Answer

Thank you for your enquiry. This antibody does not cross react with MHC class I heavy chain.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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