PE Anti-beta 2 Microglobulin antibody [B2M-01] (ab49424)
Key features and details
- PE Mouse monoclonal [B2M-01] to beta 2 Microglobulin
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
PE Anti-beta 2 Microglobulin antibody [B2M-01]
See all beta 2 Microglobulin primary antibodies -
Description
PE Mouse monoclonal [B2M-01] to beta 2 Microglobulin -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
This antibody reacts with beta 2 microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 microglobulin. Beta 2 mircoglobulin is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rabbit, Chicken, Cow, Dog -
Immunogen
Full length native protein (purified) (Human)
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Positive control
- Peripheral blood lymphocytes.
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General notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
B2M-01 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
Sequence similarities
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Post-translational
modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients. -
Cellular localization
Secreted. Detected in serum and urine. - Information by UniProt
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Database links
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- Unigene: 534255 Human
- Unigene: 702947 Human
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Alternative names
- B2M antibody
- B2MG_HUMAN antibody
- Beta 2 microglobin antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab49424 has been referenced in 2 publications.
- Khurana M et al. Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol 21:1257-65 (2006). PubMed: 16810512
- Hilgert I et al. The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) 30:369-76 (1984). PubMed: 6394369