Anti-beta 2 Microglobulin antibody [B2M-01] (PerCP/Cy5.5®) (ab157311)

Overview

  • Product name
    Anti-beta 2 Microglobulin antibody [B2M-01] (PerCP/Cy5.5®)
    See all beta 2 Microglobulin primary antibodies
  • Description
    Mouse monoclonal [B2M-01] to beta 2 Microglobulin (PerCP/Cy5.5®)
  • Host species
    Mouse
  • Conjugation
    PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
  • Specificity
    ab157311 reacts with beta 2 Microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 Microglobulin. beta 2 Microglobulin is expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rabbit, Chicken, Cow, Dog
  • Immunogen

    Purified Human beta 2 Microglobulin.

  • Positive control
    • FC: Peripheral blood lymphocytes (Negative control: DAUDI Human Burkitt lymphoma cell line, erythrocytes).
      IP:Lysates of Human lymphoid cells.
      WB: U937 Human histiocytic lymphoma cell line or RAJI Human Burkitt lymphoma cell line (Negative control: EL4 mouse lymphoblastic lymphoma cell line)
      IHC: kidney glomeruli.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 99% PBS, 0.2% BSA
  • Concentration information loading...
  • Purity
    Size exclusion
  • Purification notes
    ab157311 is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary for use in Flow Cytometry analysis.
  • Clonality
    Monoclonal
  • Clone number
    B2M-01
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 4µl for 106 cells. (or 100 µl of whole blood).

Target

  • Function
    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Involvement in disease
    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • Sequence similarities
    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Cellular localization
    Secreted. Detected in serum and urine.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2M antibody
    • B2MG_HUMAN antibody
    • Beta 2 microglobin antibody
    • Beta 2 microglobulin antibody
    • Beta 2 microglobulin precursor antibody
    • Beta chain of mhc class 1 proteins antibody
    • Beta chain of MHC class I molecules antibody
    • Beta-2-microglobulin form pI 5.3 antibody
    • CDABP0092 antibody
    • Hdcma22p antibody
    • IMD43 antibody
    see all

References

ab157311 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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