Anti-beta 2 Microglobulin antibody [B2M-01] (PerCP/Cy5.5®) (ab157311)

Overview

  • Product name
    Anti-beta 2 Microglobulin antibody [B2M-01] (PerCP/Cy5.5®)
    See all beta 2 Microglobulin primary antibodies
  • Description
    Mouse monoclonal [B2M-01] to beta 2 Microglobulin (PerCP/Cy5.5®)
  • Host species
    Mouse
  • Conjugation
    PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
  • Specificity
    ab157311 reacts with beta 2 Microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 Microglobulin. beta 2 Microglobulin is expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
  • Tested applications
    Suitable for: Flow Cyt, IP, WB, IHC-P, ELISA, RIAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rabbit, Chicken, Cow, Dog
  • Immunogen

    Purified Human beta 2 Microglobulin.

  • Positive control
    • FC: Peripheral blood lymphocytes (Negative control: DAUDI Human Burkitt lymphoma cell line, erythrocytes).
      IP:Lysates of Human lymphoid cells.
      WB: U937 Human histiocytic lymphoma cell line or RAJI Human Burkitt lymphoma cell line (Negative control: EL4 mouse lymphoblastic lymphoma cell line)
      IHC: kidney glomeruli.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 99% PBS, 0.2% BSA
  • Concentration information loading...
  • Purity
    Size exclusion
  • Purification notes
    ab157311 is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary for use in Flow Cytometry analysis.
  • Clonality
    Monoclonal
  • Clone number
    B2M-01
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 4µl for 106 cells. (or 100 µl of whole blood).
IP Use at an assay dependent concentration. Application note: ab157311 coprecipitatesbeta 2 Microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of Human lymphoid cells.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 14 kDa. Application note: Both reducing and non-reducing conditions.
IHC-P Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration. Application note: The dissociation constant of ab157311 is 1.5 x 10-8 mol/l as determined by competitive RIA.

Target

  • Function
    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Involvement in disease
    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • Sequence similarities
    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Cellular localization
    Secreted. Detected in serum and urine.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2M antibody
    • B2MG_HUMAN antibody
    • Beta 2 microglobin antibody
    • Beta 2 microglobulin antibody
    • Beta 2 microglobulin precursor antibody
    • Beta chain of mhc class 1 proteins antibody
    • Beta chain of MHC class I molecules antibody
    • Beta-2-microglobulin form pI 5.3 antibody
    • CDABP0092 antibody
    • Hdcma22p antibody
    • IMD43 antibody
    see all

References

ab157311 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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