Anti-beta 2 Microglobulin antibody [B2M/961] - BSA and Azide free (ab212756)

Overview

  • Product name

    Anti-beta 2 Microglobulin antibody [B2M/961] - BSA and Azide free
    See all beta 2 Microglobulin primary antibodies
  • Description

    Mouse monoclonal [B2M/961] to beta 2 Microglobulin - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Non human primates
  • Immunogen

    Recombinant full length protein corresponding to Human beta 2 Microglobulin aa 1-119.
    Database link: P61769

  • Positive control

    • Human cervical carcinoma, melanoma, endometrial carcinoma, renal carcinoma, bladder carcinoma and colon carcinoma tissues; Lymphoma; HL-60 or HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab212756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

Final volume 100 µL.

IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

30 minutes at room temperature.

Target

  • Function

    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Involvement in disease

    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • Sequence similarities

    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Cellular localization

    Secreted. Detected in serum and urine.
  • Information by UniProt
  • Database links

  • Alternative names

    • B2M antibody
    • B2MG_HUMAN antibody
    • Beta 2 microglobin antibody
    • Beta 2 microglobulin antibody
    • Beta 2 microglobulin precursor antibody
    • Beta chain of mhc class 1 proteins antibody
    • Beta chain of MHC class I molecules antibody
    • Beta-2-microglobulin form pI 5.3 antibody
    • CDABP0092 antibody
    • Hdcma22p antibody
    • IMD43 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with ab212756 at 1 μg/mL.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human melanoma tissue labeling beta 2 Microglobulin with ab212756 at 1 μg/mL.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human endometrial carcinoma tissue labeling beta 2 Microglobulin with ab212756 at 1 μg/mL.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human renal carcinoma tissue labeling beta 2 Microglobulin with ab212756 at 1 μg/mL.

  • Immunohistochemical analysis of paraffin embedded, formalin fixed human cervical carcinoma tissue labeling beta 2 Microglobulin with ab212756 at 1 μg/ml dilution.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human bladder carcinoma tissue labeling beta 2 Microglobulin with ab212756 at 1 μg/mL.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling beta 2 Microglobulin with ab212756 at 1 μg/mL.

References

ab212756 has not yet been referenced specifically in any publications.

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