Recombinant
RabMAb

Recombinant Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)

Overview

  • Product name

    Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free
    See all beta 2 Microglobulin primary antibodies
  • Description

    Rabbit monoclonal [EP2978Y] to beta 2 Microglobulin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human beta 2 Microglobulin (C terminal). The exact sequence is proprietary.
    (Peptide available as ab189068)

  • Positive control

    • WB: Raji, U937 and HeLa whole cell lysate (ab150035) and human bone marrow, rat and mouse kidney tissue lysates. ICC/IF: HeLa cells. IP: Raji cell lysate.
  • General notes

    Ab214769 is the carrier-free version of ab75853. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab214769 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab214769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.Can be blocked with beta 2 Microglobulin peptide (ab189068).

Can be blocked with beta 2 Microglobulin peptide (ab189068).

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
    • Involvement in disease

      Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
      Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
    • Sequence similarities

      Belongs to the beta-2-microglobulin family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      Glycation of Ile-21 is observed in long-term hemodialysis patients.
    • Cellular localization

      Secreted. Detected in serum and urine.
    • Information by UniProt
    • Database links

    • Alternative names

      • B2M antibody
      • B2MG_HUMAN antibody
      • Beta 2 microglobin antibody
      • Beta 2 microglobulin antibody
      • Beta 2 microglobulin precursor antibody
      • Beta chain of mhc class 1 proteins antibody
      • Beta chain of MHC class I molecules antibody
      • Beta-2-microglobulin form pI 5.3 antibody
      • CDABP0092 antibody
      • Hdcma22p antibody
      • IMD43 antibody
      see all

    Images

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling beta 2 Microglobulin with purified ab75853 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75853).

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling beta 2 Microglobulin with purified ab75853 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75853).

    • ab75853 (purified) at 1/20 immunoprecipitating beta 2 Microglobulin in Raji whole cell lysate.

      Lane 1 (input): Raji whole cell lysate (10µg)

      Lane 2 (+): ab75853 + Raji whole cell lysate (10µg).

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75853 in Raji whole cell lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75853).

    References

    This product has been referenced in:

    • Yousef H  et al. Systemic attenuation of the TGF-ß pathway by a single drug simultaneously rejuvenates hippocampal neurogenesis and myogenesis in the same old mammal. Oncotarget 6:11959-78 (2015). Read more (PubMed: 26003168) »
    • Sabnis H  et al. Capillary nano-immunoassay for Akt 1/2/3 and 4EBP1 phosphorylation in acute myeloid leukemia. J Transl Med 12:166 (2014). WB ; Human . Read more (PubMed: 24923301) »
    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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