Product nameAnti-beta 3 Adrenergic Receptor antibody
See all beta 3 Adrenergic Receptor primary antibodies
DescriptionChicken polyclonal to beta 3 Adrenergic Receptor
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat
- Mouse brain extract MEF1 cell line
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab59685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 23283549
ab37382 - Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.
FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
Tissue specificityExpressed mainly in adipose tissues.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.
Cellular localizationCell membrane.
- Information by UniProt
- ADR B3 antibody
- ADRB 3 antibody
- ADRB3 antibody
ICC/IF image of ab59685 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59685, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96947, DyLight® 488 goat anti-chicken IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Lee HH et al. TonEBP/NFAT5 promotes obesity and insulin resistance by epigenetic suppression of white adipose tissue beiging. Nat Commun 10:3536 (2019). Read more (PubMed: 31387996) »
- Choo MS et al. The preventive effect of a free radical scavenger on oxidative stress after the relief of partial bladder outlet obstruction in a rat model. PLoS One 13:e0199800 (2018). Read more (PubMed: 30346942) »