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  1. Link

    beta-3-adrenergic-receptor-antibody-ab94506.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Adrenergic Receptors
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Anti-beta 3 Adrenergic Receptor antibody (ab94506)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (19)

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Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
  • Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
  • Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)

Key features and details

  • Rabbit polyclonal to beta 3 Adrenergic Receptor
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat
  • Isotype: IgG

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Overview

  • Product name

    Anti-beta 3 Adrenergic Receptor antibody
    See all beta 3 Adrenergic Receptor primary antibodies
  • Description

    Rabbit polyclonal to beta 3 Adrenergic Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab108460)

  • Positive control

    • WB: Mouse brown adipose tissue lysate. IHC-P: Mouse pancreas tissue. Mouse Adipose, Mouse Bladder, Mouse ovary.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Adrenergic Receptors
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Mouse beta 3 Adrenergic Receptor peptide (ab108460)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab94506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 43 kDa).
IHC-P
Use a concentration of 1 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 43 kDa).
IHC-P
Use a concentration of 1 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
  • Tissue specificity

    Expressed mainly in adipose tissues.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P13945 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 11556 Mouse
    • Entrez Gene: 25645 Rat
    • SwissProt: P25962 Mouse
    • SwissProt: P26255 Rat
    • Unigene: 278475 Mouse
    • Unigene: 10100 Rat
    • Alternative names

      • ADR B3 antibody
      • ADRB 3 antibody
      • ADRB3 antibody
      • ADRB3_HUMAN antibody
      • ADRB3R antibody
      • Adrenergic beta 3 receptor antibody
      • B3AR antibody
      • Beta 3 adrenoceptor antibody
      • Beta 3 adrenoreceptor antibody
      • Beta 3AR antibody
      • Beta-3 adrenergic receptor antibody
      • Beta-3 adrenoceptor antibody
      • Beta-3 adrenoreceptor antibody
      • Beta3AR antibody
      • FLJ99960 antibody
      see all

    Images

    • Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
      Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
      All lanes : Anti-beta 3 Adrenergic Receptor antibody (ab94506) at 1 µg/ml

      Lane 1 : Brown Adipose (Mouse) Tissue Lysate
      Lane 2 : Brown Adipose (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 44 kDa why is the actual band size different from the predicted?


      Exposure time: 8 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab94506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)

      IHC image of beta 3 Adrenergic Receptor staining in Mouse adipose (white) tissue formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94506, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)

      IHC image of beta 3 Adrenergic Receptor staining in Mouse Ovary Normal tissue formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94506, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)

      IHC image of beta 3 Adrenergic Receptor staining in Mouse Bladder Normal tissue formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94506, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
      Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
      Anti-beta 3 Adrenergic Receptor antibody (ab94506) at 1 µg/ml + Brown Adipose (Mouse) Tissue Lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 44 kDa why is the actual band size different from the predicted?
      Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab94506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    • Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
      Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
      All lanes : Anti-beta 3 Adrenergic Receptor antibody (ab94506) at 1 µg/ml

      Lane 1 : Mouse ovary tissue lysate
      Lane 2 : Mouse bladder tissue lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 43 kDa


      Exposure time: 20 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab94506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)

      IHC image of beta 3 Adrenergic Receptor staining in Mouse pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94506, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (19)

    Publishing research using ab94506? Please let us know so that we can cite the reference in this datasheet.

    ab94506 has been referenced in 19 publications.

    • Na J  et al. The crosstalk of HDAC3, microRNA-18a and ADRB3 in the progression of heart failure. Cell Biosci 11:31 (2021). PubMed: 33549119
    • Lee HJ  et al. Deletion of adipose triglyceride lipase abolishes blood flow increase after ß3-adrenergic stimulation in visceral adipose tissue of mice. Korean J Physiol Pharmacol 25:355-363 (2021). PubMed: 34187952
    • Blondin DP  et al. Human Brown Adipocyte Thermogenesis Is Driven by ß2-AR Stimulation. Cell Metab 32:287-300.e7 (2020). PubMed: 32755608
    • Song J  et al. Upregulation of gastric norepinephrine with beta-adrenoceptors and gastric dysmotility in a rat model of functional dyspepsia. Physiol Res 69:135-143 (2020). PubMed: 31852208
    • Gordon DM  et al. Bilirubin remodels murine white adipose tissue by reshaping mitochondrial activity and the coregulator profile of peroxisome proliferator-activated receptor a. J Biol Chem 295:9804-9822 (2020). PubMed: 32404366
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-beta 3 Adrenergic Receptor antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (brain)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    40 µg
    Specification
    brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 17 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta 3 Adrenergic Receptor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Cow Cell (aortic endothelial cells)
    Permeabilization
    Yes - Saponin 0,1%
    Specification
    aortic endothelial cells
    Blocking step
    PBS/Saponin0,1%/BSA5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    PFA 4%
    Read More

    Miss. Virginie Joris

    Verified customer

    Submitted Jul 06 2015

    Question

    We would like to test your antibody ab94506 (anit-beta 3 adrenergic receptor),
    in immunofluorescence (IF) on mouse beta 3 knock-out cells. We
    already know your system of Abreview because we used it in the past.
    So, we would like to know if you can issue a discount code for testing
    this ab94506 in IF on mouse beta 3 knock-out cells and send this code to us
    by email?
    Thank your for your collaboration!
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Feb 17 2012

    Answer

    Thank you for contacting us.

    ab94506 is not tested in ICC/IF so it is eligible for testing discount. Are you planning using the knockout cells only or would like to try the normal cells also?

    I will not be able to provide you the discount code for knockout cells however will provide the discount code for normal cells that express beta 3 adrenergic receptor. Could you conform the cell line you would be testing?

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 17 2012

    Question

    I would test the Antibody on the heart cells of normal mice and heart tissue. Just wanted to say that I also mouse cells beta 3 knockout that could serve as a negative control.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 20 2012

    Answer

    DISCOUNT CODE: XXXXXXXX
    Expiration date: 19-06-2012

    I am very pleased to hear you would like to accept our offer and test ab94506 in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for [ICC/IF] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Feb 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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