Anti-beta 3 Adrenergic Receptor antibody - N-terminal (ab140713)

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Overview

  • Product name

    Anti-beta 3 Adrenergic Receptor antibody - N-terminal
    See all beta 3 Adrenergic Receptor primary antibodies

  • Description

    Rabbit polyclonal to beta 3 Adrenergic Receptor - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Monkey, Gorilla, Elephant

  • Immunogen

    Synthetic peptide, corresponding to a 20 residue sequence within N terminal (extracellular domain) amino acids of Human beta 3 Adrenergic Receptor (NP_000016.1).

  • Positive control

    • Human adipose tissue

Applications

Our Abpromise guarantee covers the use of ab140713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 17 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.

  • Tissue specificity

    Expressed mainly in adipose tissues.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ADR B3 antibody
      • ADRB 3 antibody
      • ADRB3 antibody
      • ADRB3_HUMAN antibody
      • ADRB3R antibody
      • Adrenergic beta 3 receptor antibody
      • B3AR antibody
      • Beta 3 adrenoceptor antibody
      • Beta 3 adrenoreceptor antibody
      • Beta 3AR antibody
      • Beta-3 adrenergic receptor antibody
      • Beta-3 adrenoceptor antibody
      • Beta-3 adrenoreceptor antibody
      • Beta3AR antibody
      • FLJ99960 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody - N-terminal (ab140713)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody - N-terminal (ab140713)
      Immunohistochemical analysis of formalin fixed, paraffin embedded Human adipose tissue labelling beta 3 Adrenergic Receptor with ab140713 at 17 µg/ml.

    References

    This product has been referenced in:

    • Deng J  et al. Targeting ß3-adrenergic receptor signaling inhibits neuroblastoma cell growth via suppressing the mTOR pathway. Biochem Biophys Res Commun 514:295-300 (2019). Read more (PubMed: 31030945) »
    See 1 Publication for this product

    Publishing research using ab140713? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Please recommend an anti-human adrenergic receptor beta 3 antibody for IF detection of human receptor on cultured cells, preferably with low chance of cross-reactivity with mouse.

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    Answer

    Neither of the beta 3 Adrenergic Receptor antibodies you asked about have been tested for ICC/IF, only IHC. If you do decide to try one, you will be better off with ab140709. The immunogen shares only 22% identity with the mouse protein, and the immunogen for ab140713 shares 75% identity. Reactivity with mouse has not, however, been tested by experiment.

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    Question

    I have a few questions regarding the IHC image for this product:
    1. Please confirm if the pink stain is due to IHC or eosin staining the cytoplasm?
    2. Please confirm if the blue spots are nuclei and if this staining is due to haematoxylin?
    3. If this figure is generated by IHC, what enzyme is the secondary antibody conjugated to, and what is the enzyme substrate?

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    Answer

    Q1: Please confirm if the pink stain is due to IHC or eosin staining the cytoplasm?
    A1: The slides are not stained with Eosin. The enzyme substrate used in these experiments is Vector Red which produces a fuchsia colored deposit.

    Q2: Please confirm if the blue spots are nuclei and if this staining is due to haematoxylin?
    A2: Yes, the slides are counterstained with Hamatoxylin which stains the nuclei blue.

    Q3: If this figure is generated by IHC, what enzyme is the secondary antibody conjugated to, and what is the enzyme substrate?
    A3: The image shown on our datasheet is of IHC using formalin-fixed paraffin-embedded human tissue. The enzyme used is alkaline phosphatase and the substrate is Vector Red.


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