Anti-beta 3 Adrenergic Receptor antibody - N-terminal (ab140713)

Submit a review Q&A (2)References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody - N-terminal (ab140713)

    Key features and details

    • Rabbit polyclonal to beta 3 Adrenergic Receptor - N-terminal
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-beta 3 Adrenergic Receptor antibody - N-terminal
      See all beta 3 Adrenergic Receptor primary antibodies

    • Description

      Rabbit polyclonal to beta 3 Adrenergic Receptor - N-terminal

    • Host species

      Rabbit

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Rabbit, Guinea pig, Monkey, Gorilla, Elephant

    • Immunogen

      Synthetic peptide, corresponding to a 20 residue sequence within N terminal (extracellular domain) amino acids of Human beta 3 Adrenergic Receptor (NP_000016.1).

    • Positive control

      • Human adipose tissue

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab140713 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 17 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 17 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.

    • Tissue specificity

      Expressed mainly in adipose tissues.

    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ADR B3 antibody
        • ADRB 3 antibody
        • ADRB3 antibody
        • ADRB3_HUMAN antibody
        • ADRB3R antibody
        • Adrenergic beta 3 receptor antibody
        • B3AR antibody
        • Beta 3 adrenoceptor antibody
        • Beta 3 adrenoreceptor antibody
        • Beta 3AR antibody
        • Beta-3 adrenergic receptor antibody
        • Beta-3 adrenoceptor antibody
        • Beta-3 adrenoreceptor antibody
        • Beta3AR antibody
        • FLJ99960 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody - N-terminal (ab140713)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody - N-terminal (ab140713)
        Immunohistochemical analysis of formalin fixed, paraffin embedded Human adipose tissue labelling beta 3 Adrenergic Receptor with ab140713 at 17 µg/ml.

      References (3)

      Publishing research using ab140713? Please let us know so that we can cite the reference in this datasheet.

      ab140713 has been referenced in 3 publications.

      • Bruno G  et al. ß3-adrenoreceptor blockade reduces tumor growth and increases neuronal differentiation in neuroblastoma via SK2/S1P2 modulation. Oncogene 39:368-384 (2020). PubMed: 31477835
      • Calvani M  et al. ß3-Adrenoreceptor Blockade Reduces Hypoxic Myeloid Leukemic Cells Survival and Chemoresistance. Int J Mol Sci 21:N/A (2020). PubMed: 32545695
      • Deng J  et al. Targeting ß3-adrenergic receptor signaling inhibits neuroblastoma cell growth via suppressing the mTOR pathway. Biochem Biophys Res Commun 514:295-300 (2019). PubMed: 31030945

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Question

      Please recommend an anti-human adrenergic receptor beta 3 antibody for IF detection of human receptor on cultured cells, preferably with low chance of cross-reactivity with mouse.

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      Answer

      Neither of the beta 3 Adrenergic Receptor antibodies you asked about have been tested for ICC/IF, only IHC. If you do decide to try one, you will be better off with ab140709. The immunogen shares only 22% identity with the mouse protein, and the immunogen for ab140713 shares 75% identity. Reactivity with mouse has not, however, been tested by experiment.

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      Question

      I have a few questions regarding the IHC image for this product:
      1. Please confirm if the pink stain is due to IHC or eosin staining the cytoplasm?
      2. Please confirm if the blue spots are nuclei and if this staining is due to haematoxylin?
      3. If this figure is generated by IHC, what enzyme is the secondary antibody conjugated to, and what is the enzyme substrate?

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      Answer

      Q1: Please confirm if the pink stain is due to IHC or eosin staining the cytoplasm?
      A1: The slides are not stained with Eosin. The enzyme substrate used in these experiments is Vector Red which produces a fuchsia colored deposit.

      Q2: Please confirm if the blue spots are nuclei and if this staining is due to haematoxylin?
      A2: Yes, the slides are counterstained with Hamatoxylin which stains the nuclei blue.

      Q3: If this figure is generated by IHC, what enzyme is the secondary antibody conjugated to, and what is the enzyme substrate?
      A3: The image shown on our datasheet is of IHC using formalin-fixed paraffin-embedded human tissue. The enzyme used is alkaline phosphatase and the substrate is Vector Red.


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