Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
Key features and details
- Mouse monoclonal [M117] to beta Dystroglycan (phospho Y892)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta Dystroglycan (phospho Y892) antibody [M117]
See all beta Dystroglycan primary antibodies -
Description
Mouse monoclonal [M117] to beta Dystroglycan (phospho Y892) -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human beta Dystroglycan (phospho Y892) conjugated to keyhole limpet haemocyanin.
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Positive control
- HepG2 cells or HeLa cells treated with pervanadate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M117 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab29037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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WB: 1/1000. Membrane was incubated with diluted antibody in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation. -
Cellular localization
Type I membrane protein. -
Database links
- Entrez Gene: 1605 Human
- Omim: 128239 Human
- SwissProt: Q14118 Human
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Alternative names
- 156DAG antibody
- A3a antibody
- AGRNR antibody
see all
Images
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All lanes : Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
Lane 1 : Lysates from HepG2 cells (untreated)
Lane 2 : Lysates from HepG2 cells treated with pervanadate (1 mM) for 30 min
Lysates/proteins at 20 µg per lane.
Predicted band size: 97 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Dystroglycan is expressed as a precursor protein (97kDa) that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa. -
Western blot analysis of HepG2 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). Blots were probed with anti-beta-dystroglycan (lanes 1 & 2) and anti-beta-dystroglycan (Tyr-892) (lanes 3 & 4).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab29037 has not yet been referenced specifically in any publications.