• Product name
    Anti-beta Galactosidase antibody
    See all beta Galactosidase primary antibodies
  • Description
    Rabbit polyclonal to beta Galactosidase
  • Host species
  • Specificity
    Detects recombinant beta Galactosidase in Western blotting. When used in Western blotting on 20ug Hela cell lysate dosed with 0.1ug of beta Galactosidase, detects a single strong band corresponding to beta Galactosidase.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Immunogen

    Full length native protein (purified) (E. coli).

  • Positive control
    • This antibody detects beta galactosidase in HeLa cells in WB


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab4761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 17268532
WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).


  • Relevance
    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localization
  • Alternative names
    • Beta D galactosidase antibody
    • Beta gal antibody
    • Beta galactosidase antibody
    • ECK0341 antibody
    • JW0335 antibody
    • Lactase antibody
    • lacZ antibody
    see all


  • All lanes : Anti-beta Galactosidase antibody (ab4761) at 1/1000 dilution

    Lane 1 : Beta Gal recombinant protein at 0.1 µg
    Lane 2 : 20ug HeLa WCL + 0.1ug Beta Gal recombinant protein at 20 µg

    All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 116 kDa
    Observed band size: 116 kDa

    Western blot using ab4761 at 1/1000.

    Lane 1: 0.1µg Beta Gal recombinant protein
    Lane 2: 20µg HeLa WCL + 0.1µg Beta Gal recombinant protein

    Secondary ab: Alexa Fluor Goat polyclonal to Rabbit IgG (1/5000).
    Expected size: 116kD


This product has been referenced in:
  • Clemente C  et al. MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis. Nat Commun 9:910 (2018). Read more (PubMed: 29500407) »
  • Blanco MJ  et al. Developmental expression of membrane type 4-matrix metalloproteinase (Mt4-mmp/Mmp17) in the mouse embryo. PLoS One 12:e0184767 (2017). Read more (PubMed: 28926609) »

See all 8 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH=6
Blocking step
2%BSA, 10% serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 10 2013

Code de remise : xxxxxx
Date d’expiration : xxxxxxxx
Valeur: €xxxxxx

Je suis ravie que vous souhaitiez participer a notre offre et tester ab4761 chez la souris. Le code de réduction est xxxxxxxxxx. Ce code de r&ea...

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Merci de nous avoir contactés.

L’anticorps ab4761 a été testé en IHC-P et WB. L’espèce testée en IHC-P est l’humain.

Voir la publication suivante : Hachiya A et al. Gene t...

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Merci de nous avoir contactés.

Nous n'avons pas encore testé l’anticorps ab4761 chez la souris. Cependant, puisque nous n'avons pour l'instant pas d'image IHC-P chez la souris à mettre sur la fiche technique du ab476...

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Thank you for your inquiry.
I can confirm that ab4761 will detect beta Galactosidase from E.coli origin.
If beta Galactosidase is overexpressed (ectopically expressed) in human tissue than this antibody is suitable for this.
We tested th...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case ...

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Thank you for your reply.

It is absolutely correct that you will get the full price of ab4761 as a discount off a future order.

If you would like to take part in this program, please let me know. I will be happy to issue a testing d...

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Thank you very much for your interest in ab4761 and ab616.

I confirm that we cannot guarantee ab616 for IHC and we also do not recommend this antibody for this application.

To our knowledge, ab4761 has not been tested in IHC - Who...

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Thank you for your inquiry.
I am happy to confirm that ab4761 is tested and guaranteed for IHC-P.
The following publication even used this antibody with human/ viral transfected samples:
Hachiya A et al. Gene transfer in human skin with ...

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Thank you for contacting us.

We have several antibodies against beta galactosidase, two of which have been validated for IHC staining of paraffin embedded sections. You may want to consider chicken polyclonal ab9361 or rabbit polyclonal ab47...

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