Recombinant HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab190574)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1569Y] to beta III Tubulin - Neuronal Marker
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker
See all beta III Tubulin primary antibodies -
Description
HRP Rabbit monoclonal [EP1569Y] to beta III Tubulin - Neuronal Marker -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and PC12 whole cell lysates. Mouse brain, mouse spinal cord, rat brain and rat spinal cord tissue lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1569Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab190575)
- Biotin Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab195903)
- Alexa Fluor® 594 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab201740)
- Alexa Fluor® 555 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab202519)
- Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
- APC Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab224977)
- FITC Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab224978)
- PE Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab224979)
- Alexa Fluor® 488 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab283728)
- Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab52623)
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/3000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
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Notes |
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WB
1/3000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. -
Tissue specificity
Expression is primarily restricted to central and peripheral nervous system. -
Involvement in disease
Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. -
Sequence similarities
Belongs to the tubulin family. -
Domain
The highly acidic C-terminal region may bind cations such as calcium. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 10381 Human
- Entrez Gene: 22152 Mouse
- Entrez Gene: 246118 Rat
- Omim: 602661 Human
- SwissProt: Q13509 Human
- SwissProt: Q9ERD7 Mouse
- SwissProt: Q4QRB4 Rat
- Unigene: 511743 Human
see all -
Alternative names
- beta 3 tubulin antibody
- beta 4 antibody
- beta-4 antibody
see all
Images
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Lane 1 : HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab190574) at 20 µg
Lane 2 : HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab190574) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TUBB3 (beta III Tubulin) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 2 minutesab190574 was shown to specifically react with beta III Tubulin in wild-type HAP1 cells as signal was lost in TUBB3 (beta III Tubulin) knockout cells. Wild-type and TUBB3 (beta III Tubulin) knockout samples were subjected to SDS-PAGE. Ab190574 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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All lanes : HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab190574) at 1/3000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190574 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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All lanes : HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab190574) at 1/10000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190574 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab190574 has not yet been referenced specifically in any publications.