Overview

  • Product name
    Anti-beta IV Tubulin antibody [EPR16775]
    See all beta IV Tubulin primary antibodies
  • Description
    Rabbit monoclonal [EPR16775] to beta IV Tubulin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human beta IV Tubulin aa 50-150. The exact sequence is proprietary.
    Database link: P04350

  • Positive control
    • WB: Human fetal brain, Human cerebellum and Human fetal kidney lysates; HeLa, Jurkat, Neuro-2a, C6, RAW 264.7, and PC-12 whole cell lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain and heart lysates. IHC-P: Human cerebral cortex, Mouse hippocampus and Rat thalamus tissues. ICC/IF: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF 1/100.
Flow Cyt 1/20.

Target

  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similarities
    Belongs to the tubulin family.
  • Domain
    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta 4 antibody
    • Beta 4 tubulin antibody
    • beta 5 antibody
    • beta four tubulin antibody
    • Dystonia 4 torsion (autosomal dominant) antibody
    • MC1R antibody
    • TBB4_HUMAN antibody
    • TUB B4 antibody
    • TUBB 4 antibody
    • tubb4 antibody
    • TUBB4A antibody
    • TUBB5 antibody
    • Tubulin 5 beta antibody
    • Tubulin beta 3 antibody
    • Tubulin beta 4 antibody
    • Tubulin beta 4 chain antibody
    • Tubulin beta 4A class IVa antibody
    • Tubulin beta 5 antibody
    • Tubulin beta IV antibody
    • Tubulin beta-4 chain antibody
    see all

Images

  • All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human cerebellum lysates
    Lane 3 : Human fetal kidney lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 3 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Mouse kidney lysates
    Lane 4 : Mouse spleen lysates
    Lane 5 : Rat brain lysates
    Lane 6 : Rat heart lysates
    Lane 7 : Rat kidney lysates
    Lane 8 : Rat spleen lysates
    Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta IV Tubulin with ab179504 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody (ab150077) at 1/200 dilution (green). Cytoplasm staining is observed.

    Tubulin is detected with Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue).

     

    The  negative controls:-

    -ve control 1 - ab179504 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.
    -ve control 2 -  Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human cerebral cortex issue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of cerebral cortex.

    Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Immunohistochemical analysis of paraffin embedded Mouse hippocampus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of mouse hippocampus.

    Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Immunohistochemical analysis of paraffin-embedded Rat thalamus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of rat thalamus.

    Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling beta IV Tubulin with ab179504 at 1/20 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line).

References

This product has been referenced in:
  • Royston L  et al. Viral chimeras decrypt the role of enterovirus capsid proteins in viral tropism, acid sensitivity and optimal growth temperature. PLoS Pathog 14:e1006962 (2018). Read more (PubMed: 29630666) »
  • Essaidi-Laziosi M  et al. Propagation of respiratory viruses in human airway epithelia reveals persistent virus-specific signatures. J Allergy Clin Immunol 141:2074-2084 (2018). ICC/IF . Read more (PubMed: 28797733) »
See all 2 Publications for this product

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