Anti-beta Actin antibody [8F10-G10] (ab170325)

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Key features and details

  • Mouse monoclonal [8F10-G10] to beta Actin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Monkey, Zebrafish, African green monkey, Chinese hamster, Pichia pastoris
  • Isotype: IgG2b

Overview

  • Product name

    Anti-beta Actin antibody [8F10-G10]
    See all beta Actin primary antibodies

  • Description

    Mouse monoclonal [8F10-G10] to beta Actin

  • Host species

    Mouse

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Monkey, Zebrafish, African green monkey, Chinese hamster, Pichia pastoris

  • Immunogen

    Full length protein corresponding to Human beta Actin. Expressed in E.coli.
    Database link: NM_001101

  • Positive control

    • WB: COS7, K562, HeLa, 3T3 cell lysate.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab170325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 42 kDa.

Target

Images

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution

    Lane 1 : COS7 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : 3T3 cell lysate

    Predicted band size: 42 kDa

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/10000 dilution

    Lane 1 : Goat muscle cell lysate
    Lane 2 : CHO-K1 cell lysate
    Lane 3 : COS7 cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : 3T3 cell lysate
    Lane 6 : K562 cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes :

    Lane 1 : Rabbit spleen cell lysate
    Lane 2 : Rabbit colon cell lysate
    Lane 3 : Rabbit skeletal muscle cell lysate
    Lane 4 : Rabbit liver cell lysate
    Lane 5 : Rabbit heart cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Lane 1 : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
    Lane 2 : Anti-beta Actin antibody [8F10-G10] (ab170325)

    All lanes : Zebrafish row cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution

    Lane 1 : Arabidopsis cell lysate at 30 µg
    Lane 2 : Arabidopsis cell lysate at 15 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution

    Lane 1 : S. cerevisiae cell lysate at 10 µg
    Lane 2 : P. pastoris cell lysate at 10 µg
    Lane 3 : S. cerevisiae cell lysate at 5 µg
    Lane 4 : P. pastoris cell lysate at 5 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes :

    Lane 1 : COS7 cell lysate
    Lane 2 : 3T3 cell lysate
    Lane 3 : k562 cell lysate
    Lane 4 : HeLa cell lysate

    Predicted band size: 42 kDa

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution

    Lane 1 : Drosophila melanogaster cell lysate at 40 µg
    Lane 2 : Drosophila melanogaster cell lysate at 20 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (6)

Publishing research using ab170325? Please let us know so that we can cite the reference in this datasheet.

ab170325 has been referenced in 6 publications.

  • Shah MK  et al. Influence of Inherent Mechanophenotype on Competitive Cellular Adherence. Ann Biomed Eng 45:2036-2047 (2017). PubMed: 28447179
  • Armstrong MC  et al. Bypass of Activation Loop Phosphorylation by Aspartate 836 in Activation of the Endoribonuclease Activity of Ire1. Mol Cell Biol 37:N/A (2017). WB ; Saccharomyces cerevisiae . PubMed: 28559428
  • Jantaruk P  et al. Potential role of an antimicrobial peptide, KLK in inhibiting lipopolysaccharide-induced macrophage inflammation. PLoS One 12:e0183852 (2017). PubMed: 28850608
  • Ji QQ  et al. Self-protective responses to norvaline-induced stress in a leucyl-tRNA synthetase editing-deficient yeast strain. Nucleic Acids Res 45:7367-7381 (2017). WB . PubMed: 28575390
  • Weaver PF  et al. Hypersalinity drives physiological and morphological changes in Limia perugiae (Poeciliidae). Biol Open 5:1093-101 (2016). WB . PubMed: 27402966
  • Magen I  et al. Intranasal NAP (davunetide) decreases tau hyperphosphorylation and moderately improves behavioral deficits in mice overexpressing a-synuclein. Pharmacol Res Perspect 2:e00065 (2014). PubMed: 25505609

Customer reviews and Q&As

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Western blot abreview for Anti-beta Actin antibody [8F10G10]

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Nov 25 2014

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