Anti-beta Actin antibody [8F10-G10] (ab170325)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-beta Actin antibody [8F10-G10]
See all beta Actin primary antibodies -
DescriptionMouse monoclonal [8F10-G10] to beta Actin
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Host speciesMouse
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Monkey, Zebrafish, African green monkey, Chinese hamster, Pichia pastoris
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Immunogen
Recombinant fragment corresponding to Human beta Actin. Expressed in E.coli.
Database link: NM_001101 -
Positive control
- WB: COS7, K562, HeLa, 3T3 cell lysate.
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General notes
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituent: 50% Glycerol -
Concentration information loading... -
PurityConcentrated Culture Supernatant
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ClonalityMonoclonal
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Clone number8F10-G10
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IsotypeIgG2b
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab170325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Predicted molecular weight: 42 kDa. |
Target
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FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
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Sequence similaritiesBelongs to the actin family.
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Post-translational
modificationsISGylated. -
Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Entrez Gene: 57934 Zebrafish
- Omim: 102630 Human
- SwissProt: P48975 Chinese hamster
- SwissProt: P60709 Human
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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![Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)](https://www.abcam.com/ps/products/170/ab170325/Images/ab170325-316808-anti-beta-actin-antibody-8f10-g10-western-blot.jpg)
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1 : COS7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : C6 cell lysate
Lane 4 : 3T3 cell lysate
Predicted band size: 42 kDa -
![Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)](https://www.abcam.com/ps/products/170/ab170325/Images/ab170325-207573-anti-beta-actin-antibody-8f10-g10-western-blot.jpg)
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/10000 dilution
Lane 1 : Goat muscle cell lysate
Lane 2 : CHO-K1 cell lysate
Lane 3 : COS7 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : 3T3 cell lysate
Lane 6 : K562 cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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![Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)](https://www.abcam.com/ps/products/170/ab170325/Images/ab170325-207576-anti-beta-actin-antibody-8f10-g10-western-blot.jpg)
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)All lanes :
Lane 1 : Rabbit spleen cell lysate
Lane 2 : Rabbit colon cell lysate
Lane 3 : Rabbit skeletal muscle cell lysate
Lane 4 : Rabbit liver cell lysate
Lane 5 : Rabbit heart cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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![Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)](https://www.abcam.com/ps/products/170/ab170325/Images/ab170325-207578-anti-beta-actin-antibody-8f10-g10-western-blot.jpg)
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)Lane 1 : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
Lane 2 : Anti-beta Actin antibody [8F10-G10] (ab170325)
All lanes : Zebrafish row cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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![Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)](https://www.abcam.com/ps/products/170/ab170325/Images/ab170325-207575-anti-beta-actin-antibody-8f10-g10-western-blot.jpg)
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution
Lane 1 : Arabidopsis cell lysate at 30 µg
Lane 2 : Arabidopsis cell lysate at 15 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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![Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)](https://www.abcam.com/ps/products/170/ab170325/Images/ab170325-207574-anti-beta-actin-antibody-8f10-g10-western-blot.jpg)
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1 : S. cerevisiae cell lysate at 10 µg
Lane 2 : P. pastoris cell lysate at 10 µg
Lane 3 : S. cerevisiae cell lysate at 5 µg
Lane 4 : P. pastoris cell lysate at 5 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)](https://www.abcam.com/ps/products/170/ab170325/Images/ab170325-169792-anti-beta-actin-antibody-8f10-g10-western-blot.jpg)
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)All lanes :
Lane 1 : COS7 cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : k562 cell lysate
Lane 4 : HeLa cell lysate
Predicted band size: 42 kDa -
![Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)](https://www.abcam.com/ps/products/170/ab170325/Images/ab170325-207577-anti-beta-actin-antibody-8f10-g10-western-blot.jpg)
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
Lane 1 : Drosophila melanogaster cell lysate at 40 µg
Lane 2 : Drosophila melanogaster cell lysate at 20 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
References
This product has been referenced in:
- Shah MK et al. Influence of Inherent Mechanophenotype on Competitive Cellular Adherence. Ann Biomed Eng 45:2036-2047 (2017). Read more (PubMed: 28447179) »
- Armstrong MC et al. Bypass of Activation Loop Phosphorylation by Aspartate 836 in Activation of the Endoribonuclease Activity of Ire1. Mol Cell Biol 37:N/A (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 28559428) »
