Name: Anti-beta Actin antibody [8F10-G10]
SKU: ab170325
Rating: 4 (2 reviews)

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Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)

Key features and details

Mouse monoclonal [8F10-G10] to beta Actin
Suitable for: WB
Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish, African green monkey, Chinese hamster
Isotype: IgG2b

Product name

Anti-beta Actin antibody [8F10-G10]
See all beta Actin primary antibodies
Description

Mouse monoclonal [8F10-G10] to beta Actin
Host species

Mouse
Tested applications

Suitable for: WBmore details
Species reactivity

Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish, African green monkey, Chinese hamster
Immunogen

Recombinant fragment corresponding to Human beta Actin. Expressed in E.coli.
Database link: P60709
Positive control
- WB: COS7, K562, HeLa, 3T3 cell lysate.
General notes

This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol
Concentration information loading...
Purity

Concentrated Culture Supernatant
Clonality

Monoclonal
Clone number

8F10-G10
Isotype

IgG2b
Research areas

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
Positive Controls
Recombinant Protein
- Recombinant Human beta Actin protein (His tag) (ab240844)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(2)	1/5000. Predicted molecular weight: 42 kDa.

Notes
WB 1/5000. Predicted molecular weight: 42 kDa.

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in disease

Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
Sequence similarities

Belongs to the actin family.
Post-translational
modifications

ISGylated.
Cellular localization

Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Target information above from: UniProt accession P60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Entrez Gene: 57934 Zebrafish
- Omim: 102630 Human
- SwissProt: P48975 Chinese hamster
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: P29751 Rabbit
- SwissProt: P60711 Rat
- SwissProt: Q7ZVI7 Zebrafish
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
- Unigene: 94978 Rat
- Unigene: 35143 Zebrafish
see all
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
- ACTB_HUMAN antibody
- Actin beta antibody
- Actin cytoplasmic 1 antibody
- Actin, cytoplasmic 1, N-terminally processed antibody
- Actx antibody
- b actin antibody
- b-actin antibody
- Beta cytoskeletal actin antibody
- Beta-actin antibody
- BRWS1 antibody
- E430023M04Rik antibody
- Melanoma X actin antibody
- MGC128179 antibody
- PS1TP5 binding protein 1 antibody
- PS1TP5BP1 antibody
see all

Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)

All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution

Lane 1 : COS7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : C6 cell lysate
Lane 4 : 3T3 cell lysate

Predicted band size: 42 kDa
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)

All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/10000 dilution

Lane 1 : Goat muscle cell lysate
Lane 2 : CHO-K1 cell lysate
Lane 3 : COS7 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : 3T3 cell lysate
Lane 6 : K562 cell lysate

Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

This image was produced with antibody produced in ascites.
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)

All lanes :

Lane 1 : Rabbit spleen cell lysate
Lane 2 : Rabbit colon cell lysate
Lane 3 : Rabbit skeletal muscle cell lysate
Lane 4 : Rabbit liver cell lysate
Lane 5 : Rabbit heart cell lysate

Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

This image was produced with antibody produced in ascites.
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)

Lane 1 : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
Lane 2 : Anti-beta Actin antibody [8F10-G10] (ab170325)

All lanes : Zebrafish row cell lysate

Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

This image was produced with antibody produced in ascites.
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)

All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution

Lane 1 : Arabidopsis cell lysate at 30 µg
Lane 2 : Arabidopsis cell lysate at 15 µg

Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

This image was produced with antibody produced in ascites.
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)

All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution

Lane 1 : S. cerevisiae cell lysate at 10 µg
Lane 2 : P. pastoris cell lysate at 10 µg
Lane 3 : S. cerevisiae cell lysate at 5 µg
Lane 4 : P. pastoris cell lysate at 5 µg

Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)

All lanes :

Lane 1 : COS7 cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : k562 cell lysate
Lane 4 : HeLa cell lysate

Predicted band size: 42 kDa
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)

All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution

Lane 1 : Drosophila melanogaster cell lysate at 40 µg
Lane 2 : Drosophila melanogaster cell lysate at 20 µg

Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab170325? Please let us know so that we can cite the reference in this datasheet.

ab170325 has been referenced in 12 publications.

Lins BB et al. Long-Term Angiotensin II Infusion Induces Oxidative and Endoplasmic Reticulum Stress and Modulates Na+ Transporters Through the Nephron. Front Physiol 12:642752 (2021). PubMed: 33868007
Zavala MR et al. Thioredoxin 1 (TRX1) Overexpression Cancels the Slow Force Response (SFR) Development. Front Cardiovasc Med 8:622583 (2021). PubMed: 33718450
Meers C et al. Genetic Characterization of Three Distinct Mechanisms Supporting RNA-Driven DNA Repair and Modification Reveals Major Role of DNA Polymerase ?. Mol Cell 79:1037-1050.e5 (2020). PubMed: 32882183
Cardoso da Silva R et al. Active transcription and Orc1 drive chromatin association of the AAA+ ATPase Pch2 during meiotic G2/prophase. PLoS Genet 16:e1008905 (2020). PubMed: 32569318
Brown M et al. Endoplasmic reticulum stress causes insulin resistance by inhibiting delivery of newly synthesized insulin receptors to the cell surface. Mol Biol Cell 31:2597-2629 (2020). PubMed: 32877278

View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Application

Western blot

Sample

Batrachochytrium dendrobatidis Cell lysate - whole cell (Zoospores)

Gel Running Conditions

Non-reduced Denaturing (Run in capillary on WES automated machine)

Loading amount

150000 cells

Specification

Zoospores

Blocking step

Antibody diluent 2 as blocking agent for 5 minute(s) · Concentration: 100%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Andrew Swafford

Verified customer

Submitted Jun 23 2022

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing

Sample

Mouse Cell lysate - whole cell (liver)

Specification

liver

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 25 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (12)

Customer reviews and Q&As

Western blot abreview for Anti-beta Actin antibody [8F10-G10]

Western blot abreview for Anti-beta Actin antibody [8F10G10]

Post-translational
modifications