Anti-beta Actin antibody [8F10-G10] (ab170325)
Key features and details
- Mouse monoclonal [8F10-G10] to beta Actin
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish, African green monkey, Chinese hamster
- Isotype: IgG2b
Overview
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Product name
Anti-beta Actin antibody [8F10-G10]
See all beta Actin primary antibodies -
Description
Mouse monoclonal [8F10-G10] to beta Actin -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish, African green monkey, Chinese hamster -
Immunogen
Recombinant fragment corresponding to Human beta Actin. Expressed in E.coli.
Database link: P60709 -
Positive control
- WB: COS7, K562, HeLa, 3T3 cell lysate.
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General notes
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Concentrated Culture Supernatant -
Clonality
Monoclonal -
Clone number
8F10-G10 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/5000. Predicted molecular weight: 42 kDa.
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Notes |
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WB
1/5000. Predicted molecular weight: 42 kDa. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Entrez Gene: 57934 Zebrafish
- Omim: 102630 Human
- SwissProt: P48975 Chinese hamster
- SwissProt: P60709 Human
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1 : COS7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : C6 cell lysate
Lane 4 : 3T3 cell lysate
Predicted band size: 42 kDa -
All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/10000 dilution
Lane 1 : Goat muscle cell lysate
Lane 2 : CHO-K1 cell lysate
Lane 3 : COS7 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : 3T3 cell lysate
Lane 6 : K562 cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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All lanes :
Lane 1 : Rabbit spleen cell lysate
Lane 2 : Rabbit colon cell lysate
Lane 3 : Rabbit skeletal muscle cell lysate
Lane 4 : Rabbit liver cell lysate
Lane 5 : Rabbit heart cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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Lane 1 : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
Lane 2 : Anti-beta Actin antibody [8F10-G10] (ab170325)
All lanes : Zebrafish row cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution
Lane 1 : Arabidopsis cell lysate at 30 µg
Lane 2 : Arabidopsis cell lysate at 15 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1 : S. cerevisiae cell lysate at 10 µg
Lane 2 : P. pastoris cell lysate at 10 µg
Lane 3 : S. cerevisiae cell lysate at 5 µg
Lane 4 : P. pastoris cell lysate at 5 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
All lanes :
Lane 1 : COS7 cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : k562 cell lysate
Lane 4 : HeLa cell lysate
Predicted band size: 42 kDa -
All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
Lane 1 : Drosophila melanogaster cell lysate at 40 µg
Lane 2 : Drosophila melanogaster cell lysate at 20 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab170325 has been referenced in 12 publications.
- Lins BB et al. Long-Term Angiotensin II Infusion Induces Oxidative and Endoplasmic Reticulum Stress and Modulates Na+ Transporters Through the Nephron. Front Physiol 12:642752 (2021). PubMed: 33868007
- Zavala MR et al. Thioredoxin 1 (TRX1) Overexpression Cancels the Slow Force Response (SFR) Development. Front Cardiovasc Med 8:622583 (2021). PubMed: 33718450
- Meers C et al. Genetic Characterization of Three Distinct Mechanisms Supporting RNA-Driven DNA Repair and Modification Reveals Major Role of DNA Polymerase ?. Mol Cell 79:1037-1050.e5 (2020). PubMed: 32882183
- Cardoso da Silva R et al. Active transcription and Orc1 drive chromatin association of the AAA+ ATPase Pch2 during meiotic G2/prophase. PLoS Genet 16:e1008905 (2020). PubMed: 32569318
- Brown M et al. Endoplasmic reticulum stress causes insulin resistance by inhibiting delivery of newly synthesized insulin receptors to the cell surface. Mol Biol Cell 31:2597-2629 (2020). PubMed: 32877278