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  1. Link

    beta-actin-antibody-8f10-g10-ab170325.pdf

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Isotype/Loading Controls Loading Controls Beta Actin
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Anti-beta Actin antibody [8F10-G10] (ab170325)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (12)

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Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)

Key features and details

  • Mouse monoclonal [8F10-G10] to beta Actin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish, African green monkey, Chinese hamster
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-beta Actin antibody [8F10-G10]
    See all beta Actin primary antibodies
  • Description

    Mouse monoclonal [8F10-G10] to beta Actin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish, African green monkey, Chinese hamster
  • Immunogen

    Recombinant fragment corresponding to Human beta Actin. Expressed in E.coli.
    Database link: P60709

  • Positive control

    • WB: COS7, K562, HeLa, 3T3 cell lysate.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin 300
    Constituent: 50% Glycerol
  • Concentration information loading...
  • Purity

    Concentrated Culture Supernatant
  • Clonality

    Monoclonal
  • Clone number

    8F10-G10
  • Isotype

    IgG2b
  • Research areas

    • Isotype/Loading Controls
    • Loading Controls
    • Beta Actin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human beta Actin protein (His tag) (ab240844)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/5000. Predicted molecular weight: 42 kDa.
Notes
WB
1/5000. Predicted molecular weight: 42 kDa.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 60 Human
    • Entrez Gene: 11461 Mouse
    • Entrez Gene: 100009272 Rabbit
    • Entrez Gene: 81822 Rat
    • Entrez Gene: 57934 Zebrafish
    • Omim: 102630 Human
    • SwissProt: P48975 Chinese hamster
    • SwissProt: P60709 Human
    • SwissProt: P60710 Mouse
    • SwissProt: P29751 Rabbit
    • SwissProt: P60711 Rat
    • SwissProt: Q7ZVI7 Zebrafish
    • Unigene: 520640 Human
    • Unigene: 708120 Human
    • Unigene: 727576 Human
    • Unigene: 328431 Mouse
    • Unigene: 391967 Mouse
    • Unigene: 94978 Rat
    • Unigene: 35143 Zebrafish
    see all
  • Alternative names

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Images

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution

    Lane 1 : COS7 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : 3T3 cell lysate

    Predicted band size: 42 kDa

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/10000 dilution

    Lane 1 : Goat muscle cell lysate
    Lane 2 : CHO-K1 cell lysate
    Lane 3 : COS7 cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : 3T3 cell lysate
    Lane 6 : K562 cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes :

    Lane 1 : Rabbit spleen cell lysate
    Lane 2 : Rabbit colon cell lysate
    Lane 3 : Rabbit skeletal muscle cell lysate
    Lane 4 : Rabbit liver cell lysate
    Lane 5 : Rabbit heart cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Lane 1 : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
    Lane 2 : Anti-beta Actin antibody [8F10-G10] (ab170325)

    All lanes : Zebrafish row cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution

    Lane 1 : Arabidopsis cell lysate at 30 µg
    Lane 2 : Arabidopsis cell lysate at 15 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution

    Lane 1 : S. cerevisiae cell lysate at 10 µg
    Lane 2 : P. pastoris cell lysate at 10 µg
    Lane 3 : S. cerevisiae cell lysate at 5 µg
    Lane 4 : P. pastoris cell lysate at 5 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes :

    Lane 1 : COS7 cell lysate
    Lane 2 : 3T3 cell lysate
    Lane 3 : k562 cell lysate
    Lane 4 : HeLa cell lysate

    Predicted band size: 42 kDa

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution

    Lane 1 : Drosophila melanogaster cell lysate at 40 µg
    Lane 2 : Drosophila melanogaster cell lysate at 20 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (12)

Publishing research using ab170325? Please let us know so that we can cite the reference in this datasheet.

ab170325 has been referenced in 12 publications.

  • Lins BB  et al. Long-Term Angiotensin II Infusion Induces Oxidative and Endoplasmic Reticulum Stress and Modulates Na+ Transporters Through the Nephron. Front Physiol 12:642752 (2021). PubMed: 33868007
  • Zavala MR  et al. Thioredoxin 1 (TRX1) Overexpression Cancels the Slow Force Response (SFR) Development. Front Cardiovasc Med 8:622583 (2021). PubMed: 33718450
  • Meers C  et al. Genetic Characterization of Three Distinct Mechanisms Supporting RNA-Driven DNA Repair and Modification Reveals Major Role of DNA Polymerase ?. Mol Cell 79:1037-1050.e5 (2020). PubMed: 32882183
  • Cardoso da Silva R  et al. Active transcription and Orc1 drive chromatin association of the AAA+ ATPase Pch2 during meiotic G2/prophase. PLoS Genet 16:e1008905 (2020). PubMed: 32569318
  • Brown M  et al. Endoplasmic reticulum stress causes insulin resistance by inhibiting delivery of newly synthesized insulin receptors to the cell surface. Mol Biol Cell 31:2597-2629 (2020). PubMed: 32877278
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-beta Actin antibody [8F10-G10]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Batrachochytrium dendrobatidis Cell lysate - whole cell (Zoospores)
Gel Running Conditions
Non-reduced Denaturing (Run in capillary on WES automated machine)
Loading amount
150000 cells
Specification
Zoospores
Blocking step
Antibody diluent 2 as blocking agent for 5 minute(s) · Concentration: 100%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Andrew Swafford

Verified customer

Submitted Jun 23 2022

Western blot abreview for Anti-beta Actin antibody [8F10G10]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 25 2014

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