Overview

  • Product name
    Anti-beta Actin antibody [8F10-G10]
    See all beta Actin primary antibodies
  • Description
    Mouse monoclonal [8F10-G10] to beta Actin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Monkey, Zebrafish, African green monkey, Chinese hamster, Pichia pastoris
  • Immunogen

    Recombinant fragment corresponding to Human beta Actin. Expressed in E.coli.
    Database link: NM_001101

  • Positive control
    • WB: COS7, K562, HeLa, 3T3 cell lysate.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab170325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 42 kDa.

Target

Images

  • All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution

    Lane 1 : COS7 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : 3T3 cell lysate

    Predicted band size: 42 kDa

  • All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/10000 dilution

    Lane 1 : Goat muscle cell lysate
    Lane 2 : CHO-K1 cell lysate
    Lane 3 : COS7 cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : 3T3 cell lysate
    Lane 6 : K562 cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • All lanes :

    Lane 1 : Rabbit spleen cell lysate
    Lane 2 : Rabbit colon cell lysate
    Lane 3 : Rabbit skeletal muscle cell lysate
    Lane 4 : Rabbit liver cell lysate
    Lane 5 : Rabbit heart cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Lane 1 : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
    Lane 2 : Anti-beta Actin antibody [8F10-G10] (ab170325)

    All lanes : Zebrafish row cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution

    Lane 1 : Arabidopsis cell lysate at 30 µg
    Lane 2 : Arabidopsis cell lysate at 15 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution

    Lane 1 : S. cerevisiae cell lysate at 10 µg
    Lane 2 : P. pastoris cell lysate at 10 µg
    Lane 3 : S. cerevisiae cell lysate at 5 µg
    Lane 4 : P. pastoris cell lysate at 5 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • All lanes :

    Lane 1 : COS7 cell lysate
    Lane 2 : 3T3 cell lysate
    Lane 3 : k562 cell lysate
    Lane 4 : HeLa cell lysate

    Predicted band size: 42 kDa

  • All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution

    Lane 1 : Drosophila melanogaster cell lysate at 40 µg
    Lane 2 : Drosophila melanogaster cell lysate at 20 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Shah MK  et al. Influence of Inherent Mechanophenotype on Competitive Cellular Adherence. Ann Biomed Eng 45:2036-2047 (2017). Read more (PubMed: 28447179) »
  • Armstrong MC  et al. Bypass of Activation Loop Phosphorylation by Aspartate 836 in Activation of the Endoribonuclease Activity of Ire1. Mol Cell Biol 37:N/A (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 28559428) »
See all 6 Publications for this product

Customer reviews and Q&As

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Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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Verified customer

Submitted Nov 25 2014

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