Anti-beta Actin antibody (ab119716)
Key features and details
- Rabbit polyclonal to beta Actin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-beta Actin antibody
See all beta Actin primary antibodies -
Description
Rabbit polyclonal to beta Actin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to amino acids at the C-terminal of Human beta Actin.
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Positive control
- HeLa, 293T, PC12 and NIH3T3 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49% HEPES, 0.88% Sodium chloride, 0.01% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab119716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Predicted molecular weight: 42 kDa. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: P60711 Rat
- Unigene: 520640 Human
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - Anti-beta Actin antibody (ab119716)All lanes : Anti-beta Actin antibody (ab119716) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH3T3 cell lysate
Predicted band size: 42 kDa
References (29)
ab119716 has been referenced in 29 publications.
- Chudnovets A et al. Dose-dependent structural and immunological changes in the placenta and fetal brain in response to systemic inflammation during pregnancy. Am J Reprod Immunol 84:e13248 (2020). PubMed: 32306461
- Sanchez E et al. Syntaxin 3, but not syntaxin 4, is required for mast cell-regulated exocytosis, where it plays a primary role mediating compound exocytosis. J Biol Chem 294:3012-3023 (2019). PubMed: 30563839
- He J et al. Interleukin-9 promotes tumorigenesis through augmenting angiogenesis in non-small cell lung cancer. Int Immunopharmacol 75:105766 (2019). PubMed: 31352324
- Pan Y et al. Reduced eIF3d accelerates HIV disease progression by attenuating CD8+ T cell function. J Transl Med 17:167 (2019). PubMed: 31118081
- Naji S et al. Cadmium Induces Migration of Colon Cancer Cells: Roles of Reactive Oxygen Species, P38 and Cyclooxygenase-2. Cell Physiol Biochem 52:1517-1534 (2019). PubMed: 31120230
