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    beta-actin-antibody-ab119716.pdf

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Isotype/Loading Controls Loading Controls Beta Actin
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Anti-beta Actin antibody (ab119716)

  • Datasheet
  • SDS
Submit a review Submit a question References (50)

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Western blot - Anti-beta Actin antibody (ab119716)

    Key features and details

    • Rabbit polyclonal to beta Actin
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-beta Actin antibody [SP124] (ab115777)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-beta Actin antibody
      See all beta Actin primary antibodies
    • Description

      Rabbit polyclonal to beta Actin
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Immunogen

      Synthetic peptide corresponding to amino acids at the C-terminal of Human beta Actin.

    • Positive control

      • HeLa, 293T, PC12 and NIH3T3 cell lysates.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      Preservative: 0.03% Sodium azide
      Constituents: 49% HEPES, 0.88% Sodium chloride, 0.01% BSA, 50% Glycerol
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Isotype/Loading Controls
      • Loading Controls
      • Beta Actin
      • Tags & Cell Markers
      • Subcellular Markers
      • Cytoskeleton
      • Actin
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human beta Actin protein (His tag) (ab240844)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab119716 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/5000. Predicted molecular weight: 42 kDa.
    Notes
    WB
    1/5000. Predicted molecular weight: 42 kDa.

    Target

    • Function

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in disease

      Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
    • Sequence similarities

      Belongs to the actin family.
    • Post-translational
      modifications

      ISGylated.
    • Cellular localization

      Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Target information above from: UniProt accession P60709 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 60 Human
      • Entrez Gene: 11461 Mouse
      • Entrez Gene: 81822 Rat
      • Omim: 102630 Human
      • SwissProt: P60709 Human
      • SwissProt: P60710 Mouse
      • SwissProt: P60711 Rat
      • Unigene: 520640 Human
      • Unigene: 708120 Human
      • Unigene: 727576 Human
      • Unigene: 328431 Mouse
      • Unigene: 391967 Mouse
      • Unigene: 94978 Rat
      see all
    • Alternative names

      • A26C1A antibody
      • A26C1B antibody
      • ACTB antibody
      • ACTB_HUMAN antibody
      • Actin beta antibody
      • Actin cytoplasmic 1 antibody
      • Actin, cytoplasmic 1, N-terminally processed antibody
      • Actx antibody
      • b actin antibody
      • b-actin antibody
      • Beta cytoskeletal actin antibody
      • Beta-actin antibody
      • BRWS1 antibody
      • E430023M04Rik antibody
      • Melanoma X actin antibody
      • MGC128179 antibody
      • PS1TP5 binding protein 1 antibody
      • PS1TP5BP1 antibody
      see all

    Images

    • Western blot - Anti-beta Actin antibody (ab119716)
      Western blot - Anti-beta Actin antibody (ab119716)
      All lanes : Anti-beta Actin antibody (ab119716) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NIH3T3 cell lysate

      Predicted band size: 42 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (50)

    Publishing research using ab119716? Please let us know so that we can cite the reference in this datasheet.

    ab119716 has been referenced in 50 publications.

    • Mohseni R  et al. Therapeutic effects of Chlorella vulgaris on carbon tetrachloride induced liver fibrosis by targeting Hippo signaling pathway and AMPK/FOXO1 axis. Mol Biol Rep 48:117-126 (2021). PubMed: 33296068
    • Cong X  et al. Silencing GnT-V reduces oxaliplatin chemosensitivity in human colorectal cancer cells through N-glycan alteration of organic cation transporter member 2. Exp Ther Med 21:128 (2021). PubMed: 33376510
    • Zhang P  et al. microRNA-130b downregulation potentiates chondrogenic differentiation of bone marrow mesenchymal stem cells by targeting SOX9. Braz J Med Biol Res 54:e10345 (2021). PubMed: 33624729
    • Xie X  et al. Interleukin-36a suppresses growth of non-small cell lung cancer in vitro by reducing angiogenesis. FEBS Open Bio 11:1353-1363 (2021). PubMed: 33713575
    • Yang X  et al. Schistosoma japonicum Infection Leads to the Reprogramming of Glucose and Lipid Metabolism in the Colon of Mice. Front Vet Sci 8:645807 (2021). PubMed: 33791356
    View all Publications for this product

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