Overview

  • Product name
  • Description
    Rabbit polyclonal to beta Actin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human beta Actin aa 1-100. The exact sequence is proprietary.
    Database link: P60709

  • Positive control
    • WB: Recombinant Human alpha smooth muscle Actin protein (ab114148), U-2 OS and SK-N-SH cell lysate. IHC-P: Human leiomyoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab16039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP Use a concentration of 5 µg/ml.

Target

Images

  • All lanes : Anti-beta Actin antibody (ab16039) at 1 µg/ml

    Lane 1 : SK-N-SH (human neuroblastoma) whole cell lysate
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : U-2 OS (human osteosarcoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Formalin-fixed, paraffin-embedded human leiomyoma tissue stained for beta Actin with ab16039 (1/100 dilution) in immunohistochemical analysis.

  • beta Actin was immunoprecipitated using 0.5mg SK-N-SH (human neuroblastoma cell line) whole cell extract, 5 µg of Rabbit polyclonal to beta Actin and 50 µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10 minutes, SK-N-SH whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.

    Proteins were eluted by addition of 40 µl SDS loading buffer and incubated for 10 minutes at 70°C; 10 µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16039.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 42kDa; beta Actin

References

This product has been referenced in:
  • Zheng XM  et al. MicroRNA-30e inhibits adhesion, migration, invasion and cell cycle progression of prostate cancer cells via inhibition of the activation of the MAPK signaling pathway by downregulating CHRM3. Int J Oncol 54:443-454 (2019). Read more (PubMed: 30483762) »
  • Li D  et al. Identification and Functional Characterization of a New Splicing Variant of EZH2 in the Central Nervous System. Int J Biol Sci 15:69-80 (2019). Read more (PubMed: 30662348) »
See all 47 Publications for this product

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