Overview

  • Product name

  • Description

    Rabbit polyclonal to beta Actin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Synthetic peptide within Zebrafish beta Actin aa 329-375. The exact sequence is proprietary. Carrier-protein conjugated synthetic peptide.
    Database link: Q7ZVI7

  • Positive control

    • Zebrafish heart, muscle and whole zebrafish tissue extracts; zebrafish tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab210083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

    • Alternative names

      • A26C1A antibody
      • A26C1B antibody
      • ACTB antibody
      • ACTB_HUMAN antibody
      • Actin beta antibody
      • Actin cytoplasmic 1 antibody
      • Actin, cytoplasmic 1, N-terminally processed antibody
      • Actx antibody
      • b actin antibody
      • b-actin antibody
      • Beta cytoskeletal actin antibody
      • Beta-actin antibody
      • BRWS1 antibody
      • E430023M04Rik antibody
      • Melanoma X actin antibody
      • MGC128179 antibody
      • PS1TP5 binding protein 1 antibody
      • PS1TP5BP1 antibody
      see all

    Images

    • All lanes : Anti-beta Actin antibody (ab210083) at 1/1000 dilution

      Lane 1 : zebrafish heart tissue extract
      Lane 2 : zebrafish muscle tissue extract
      Lane 3 : whole zebrafish tissue extract

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 42 kDa



      10% SDS-PAGE

    • Immunohistochemical analysis of paraffin-embedded zebrafish tissue labeling beta Actin with ab210083 at 1/300 dilution.

    References

    ab210083 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab210083.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up