Key features and details
- Rabbit polyclonal to beta Actin
- Suitable for: IHC-P, WB
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-beta Actin antibody
See all beta Actin primary antibodies
DescriptionRabbit polyclonal to beta Actin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Zebrafish
Synthetic peptide within Zebrafish beta Actin aa 329-375. The exact sequence is proprietary. Carrier-protein conjugated synthetic peptide.
Database link: Q7ZVI7
- Zebrafish heart, muscle and whole zebrafish tissue extracts; zebrafish tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab210083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/3000. Predicted molecular weight: 42 kDa.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
All lanes : Anti-beta Actin antibody (ab210083) at 1/1000 dilution
Lane 1 : zebrafish heart tissue extract
Lane 2 : zebrafish muscle tissue extract
Lane 3 : whole zebrafish tissue extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
Immunohistochemical analysis of paraffin-embedded zebrafish tissue labeling beta Actin with ab210083 at 1/300 dilution.
ab210083 has not yet been referenced specifically in any publications.