Key features and details
- Rabbit polyclonal to beta Actin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-beta Actin antibody
See all beta Actin primary antibodies
DescriptionRabbit polyclonal to beta Actin
SpecificityPredicted molecular weight at ~41.7kDa. A double band may be observed.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human beta Actin (internal sequence).
Database link: P60709
- WB: HeLa and HEK-293T lysates. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 41.7 kDa).|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
All lanes : Anti-beta Actin antibody (ab223353) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-294T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-rabbit IgG HRP at 1/2000 dilution
Predicted band size: 41.7 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Block: 5% milk + TBST for 1 hour at RT.
Primary incubation: 1 hour at room temperature
PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (green) using ab223353 at a dilution of 1/100 in ICC/IF (centre panel). The nuclear conterstain was DAPI (blue)(left panel). The merged images are shown in the right-hand panel.
ab223353 has not yet been referenced specifically in any publications.