Overview

  • Product name

  • Description

    Rabbit polyclonal to beta Actin
  • Host species

    Rabbit
  • Specificity

    Predicted molecular weight at ~41.7kDa. A double band may be observed.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human beta Actin (internal sequence).
    Database link: P60709

  • Positive control

    • WB: HeLa and HEK-293T lysates. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 41.7 kDa).
ICC/IF 1/100.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Images

  • All lanes : Anti-beta Actin antibody (ab223353) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-294T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP at 1/2000 dilution

    Predicted band size: 41.7 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    Block: 5% milk + TBST for 1 hour at RT.

    Primary incubation: 1 hour at room temperature

  • PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (green) using ab223353 at a dilution of 1/100 in ICC/IF (centre panel). The nuclear conterstain was DAPI (blue)(left panel). The merged images are shown in the right-hand panel.

References

ab223353 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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