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  1. Link

    beta-actin-antibody-ab227387.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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Anti-beta Actin antibody (ab227387)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab227387)
  • Western blot - Anti-beta Actin antibody (ab227387)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)
  • Western blot - Anti-beta Actin antibody (ab227387)
  • Western blot - Anti-beta Actin antibody (ab227387)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)
  • Western blot - Anti-beta Actin antibody (ab227387)
  • Western blot - Anti-beta Actin antibody (ab227387)
  • Immunoprecipitation - Anti-beta Actin antibody (ab227387)
  • Western blot - Anti-beta Actin antibody (ab227387)
  • Western blot - Anti-beta Actin antibody (ab227387)
  • Western blot - Anti-beta Actin antibody (ab227387)

Key features and details

  • Rabbit polyclonal to beta Actin
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human, Drosophila melanogaster
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-beta Actin antibody
    See all beta Actin primary antibodies
  • Description

    Rabbit polyclonal to beta Actin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
    Predicted to work with: Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human beta Actin (internal sequence). The exact sequence is proprietary.
    Database link: P60709

  • Positive control

    • WB: HEK-293T, A431, HeLa, HepG2, Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12 and Rat2 whole cell extracts; Mouse and rat liver tissue extracts; Drosophila melanogaster lysate. IP: HEK-293T whole cell extract. IHC-P: Mouse pancreas and cervix tissues; Rat liver tissue. ICC/IF: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • Mouse liver tissue lysate - total protein (ab29301)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • NIH/3T3 whole cell lysate (ab7179)
    • C2C12 whole cell lysate (ab7182)
    • RAW 264.7 whole cell lysate (ab7187)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human beta Actin protein (His tag) (ab240844)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 42 kDa.
IP
1/100 - 1/1000.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 42 kDa.
IP
1/100 - 1/1000.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396526 Chicken
    • Entrez Gene: 450133 Chimpanzee
    • Entrez Gene: 280979 Cow
    • Entrez Gene: 403580 Dog
    • Entrez Gene: 60 Human
    • Entrez Gene: 11461 Mouse
    • Entrez Gene: 100009272 Rabbit
    • Entrez Gene: 81822 Rat
    • Entrez Gene: 574285 Rhesus monkey
    • Entrez Gene: 443052 Sheep
    • Entrez Gene: 398459 Xenopus laevis
    • Omim: 102630 Human
    • SwissProt: P60706 Chicken
    • SwissProt: Q5R1X3 Chimpanzee
    • SwissProt: P60712 Cow
    • SwissProt: O18840 Dog
    • SwissProt: P60709 Human
    • SwissProt: P60710 Mouse
    • SwissProt: P29751 Rabbit
    • SwissProt: P60711 Rat
    • SwissProt: O93400 Xenopus laevis
    • Unigene: 520640 Human
    • Unigene: 708120 Human
    • Unigene: 727576 Human
    • Unigene: 328431 Mouse
    • Unigene: 391967 Mouse
    • Unigene: 94978 Rat
    • Unigene: 4138 Xenopus laevis
    see all
  • Alternative names

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)

    Paraffin-embedded mouse pancreas tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab227387)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab227387)

    0.5% Triton X-100 followed by ice-cold methanol fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (green) using ab227387 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Western blot - Anti-beta Actin antibody (ab227387)
    Western blot - Anti-beta Actin antibody (ab227387)
    All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 5 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 6 : C8D30 whole cell extract
    Lane 7 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 8 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 9 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)

    Paraffin-embedded rat liver tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-beta Actin antibody (ab227387)
    Western blot - Anti-beta Actin antibody (ab227387)
    All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-beta Actin antibody (ab227387)
    Western blot - Anti-beta Actin antibody (ab227387)
    All lanes : Anti-beta Actin antibody (ab227387) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 15 µg
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 5 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 2 µg

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)

    Paraffin-embedded mouse cervix tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-beta Actin antibody (ab227387)
    Western blot - Anti-beta Actin antibody (ab227387)
    Anti-beta Actin antibody (ab227387) at 1/10000 dilution + Drosophila melanogaster lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-beta Actin antibody (ab227387)
    Western blot - Anti-beta Actin antibody (ab227387)
    All lanes : Anti-beta Actin antibody (ab227387) at 1/15000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with beta Actin shRNA, whole cell extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Immunoprecipitation - Anti-beta Actin antibody (ab227387)
    Immunoprecipitation - Anti-beta Actin antibody (ab227387)

    beta Actin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab227387. Western blot was performed from the immunoprecipitate using ab227387. Anti-rabbit IgG was used as secondary antibody.

    Lane 1: HEK-293T whole cell extract (Input).

    Lane 2: Control IgG instead of ab227387 in HEK-293T whole cell extract.

    Lane 3: ab227387 IP in HEK-293T whole cell extract. 

     

  • Western blot - Anti-beta Actin antibody (ab227387)
    Western blot - Anti-beta Actin antibody (ab227387)
    All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-beta Actin antibody (ab227387)
    Western blot - Anti-beta Actin antibody (ab227387)
    Anti-beta Actin antibody (ab227387) at 1/20000 dilution + Mouse liver tissue extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-beta Actin antibody (ab227387)
    Western blot - Anti-beta Actin antibody (ab227387)
    Anti-beta Actin antibody (ab227387) at 1/20000 dilution + Rat liver tissue extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (76)

Publishing research using ab227387? Please let us know so that we can cite the reference in this datasheet.

ab227387 has been referenced in 76 publications.

  • Zhu X  et al. Circular RNA circANKIB1 promotes the progression of osteosarcoma by regulating miR-217/PAX3 axis. J Bone Oncol 27:100347 (2021). PubMed: 33552886
  • Cai M  et al. MRPL13 Promotes Tumor Cell Proliferation, Migration and EMT Process in Breast Cancer Through the PI3K-AKT-mTOR Pathway. Cancer Manag Res 13:2009-2024 (2021). PubMed: 33658859
  • Zhang C  et al. CircRNA circ_0006892 regulates miR-24/PHLPP2 axis to mitigate cigarette smoke extract-induced bronchial epithelial cell injury. Biotechnol Appl Biochem N/A:N/A (2021). PubMed: 33734482
  • Zhang S  et al. Activation of Toll-Like Receptor 7 Signaling Pathway in Primary Sjögren's Syndrome-Associated Thrombocytopenia. Front Immunol 12:637659 (2021). PubMed: 33767707
  • Liu H  et al. Emodin Ameliorates Renal Damage and Podocyte Injury in a Rat Model of Diabetic Nephropathy via Regulating AMPK/mTOR-Mediated Autophagy Signaling Pathway. Diabetes Metab Syndr Obes 14:1253-1266 (2021). PubMed: 33776462
View all Publications for this product

Customer reviews and Q&As

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