Anti-beta Actin antibody (ab227387)
- Datasheet
- References (12)
- Protocols
Overview
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Product nameAnti-beta Actin antibody
See all beta Actin primary antibodies -
DescriptionRabbit polyclonal to beta Actin
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human, Drosophila melanogaster
Predicted to work with: Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey
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Immunogen
Recombinant fragment within Human beta Actin (internal sequence). The exact sequence is proprietary.
Database link: P60709 -
Positive control
- WB: HEK-293T, A431, HeLa, HepG2, Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12 and Rat2 whole cell extracts; Mouse and rat liver tissue extracts; Drosophila lysate. IP: HEK-293T whole cell extract. IHC-P: Mouse pancreas and cervix tissues; Rat liver tissue. ICC/IF: HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- Mouse liver tissue lysate - total protein (ab29301)
- HeLa whole cell lysate (ab29545)
- A431 whole cell lysate (ab30132)
- NIH 3T3 whole cell lysate (ab7179)
- C2C12 whole cell lysate (ab7182)
- RAW 264.7 whole cell lysate (ab7187)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
- 293T whole cell lysate (ab95494)
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 42 kDa. | |
| IP | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
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Sequence similaritiesBelongs to the actin family.
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Post-translational
modificationsISGylated. -
Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
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Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 450133 Chimpanzee
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - Anti-beta Actin antibody (ab227387)All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 5 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 6 : C8D30 whole cell extract
Lane 7 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 8 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 9 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)Paraffin-embedded mouse pancreas tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)Paraffin-embedded rat liver tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab227387)0.5% Triton X-100 followed by ice-cold methanol fixed Hela (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (green) using ab227387 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-beta Actin antibody (ab227387)All lanes : Anti-beta Actin antibody (ab227387) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 15 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 5 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 2 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Western blot - Anti-beta Actin antibody (ab227387)Anti-beta Actin antibody (ab227387) at 1/10000 dilution + Drosophila lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)Paraffin-embedded mouse cervix tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-beta Actin antibody (ab227387)All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Western blot - Anti-beta Actin antibody (ab227387)All lanes : Anti-beta Actin antibody (ab227387) at 1/15000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with beta Actin shRNA, whole cell extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Immunoprecipitation - Anti-beta Actin antibody (ab227387)beta Actin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab227387. Western blot was performed from the immunoprecipitate using ab227387. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab227387 in HEK-293T whole cell extract.
Lane 3: ab227387 IP in HEK-293T whole cell extract.
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Western blot - Anti-beta Actin antibody (ab227387)All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Western blot - Anti-beta Actin antibody (ab227387)Anti-beta Actin antibody (ab227387) at 1/20000 dilution + Mouse liver tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Western blot - Anti-beta Actin antibody (ab227387)Anti-beta Actin antibody (ab227387) at 1/20000 dilution + Rat liver tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
References
This product has been referenced in:
- Fang L et al. Icariin inhibits cell proliferation, migration and invasion by down-regulation of microRNA-625-3p in thyroid cancer cells. Biomed Pharmacother 109:2456-2463 (2019). Read more (PubMed: 30551506) »
- Fan L et al. Sevoflurane inhibits the migration and invasion of colorectal cancer cells through regulating ERK/MMP-9 pathway by up-regulating miR-203. Eur J Pharmacol 850:43-52 (2019). Read more (PubMed: 30685432) »
