Overview

  • Product name
  • Description
    Rabbit polyclonal to beta Actin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
    Predicted to work with: Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human beta Actin (internal sequence). The exact sequence is proprietary.
    Database link: P60709

  • Positive control
    • WB: HEK-293T, A431, HeLa, HepG2, Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12 and Rat2 whole cell extracts; Mouse and rat liver tissue extracts; Drosophila lysate. IP: HEK-293T whole cell extract. IHC-P: Mouse pancreas and cervix tissues; Rat liver tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 42 kDa.
IP 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 5 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 6 : C8D30 whole cell extract
    Lane 7 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 8 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 9 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded mouse pancreas tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat liver tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.

  • 0.5% Triton X-100 followed by ice-cold methanol fixed Hela (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (green) using ab227387 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • All lanes : Anti-beta Actin antibody (ab227387) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 15 µg
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 5 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 2 µg

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Anti-beta Actin antibody (ab227387) at 1/10000 dilution + Drosophila lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded mouse cervix tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-beta Actin antibody (ab227387) at 1/15000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with beta Actin shRNA, whole cell extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • beta Actin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab227387. Western blot was performed from the immunoprecipitate using ab227387. Anti-rabbit IgG was used as secondary antibody.

    Lane 1: HEK-293T whole cell extract (Input).

    Lane 2: Control IgG instead of ab227387 in HEK-293T whole cell extract.

    Lane 3: ab227387 IP in HEK-293T whole cell extract. 

     

  • All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Anti-beta Actin antibody (ab227387) at 1/20000 dilution + Mouse liver tissue extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Anti-beta Actin antibody (ab227387) at 1/20000 dilution + Rat liver tissue extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

References

This product has been referenced in:
  • Fang L  et al. Icariin inhibits cell proliferation, migration and invasion by down-regulation of microRNA-625-3p in thyroid cancer cells. Biomed Pharmacother 109:2456-2463 (2019). Read more (PubMed: 30551506) »
  • Fan L  et al. Sevoflurane inhibits the migration and invasion of colorectal cancer cells through regulating ERK/MMP-9 pathway by up-regulating miR-203. Eur J Pharmacol 850:43-52 (2019). Read more (PubMed: 30685432) »
See all 12 Publications for this product

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