Overview

  • Product name

  • Description

    Rabbit polyclonal to beta Actin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human beta Actin. The exact sequence is proprietary.
    Database link: P60709

  • Positive control

    • IHC-P: Rat prostate and thymus gland tissues; Mouse thymus gland, pancreas and esophagus tissues. ICC/IF: HeLa cells. WB: NIH/3T3, HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900).

Properties

Applications

Our Abpromise guarantee covers the use of ab228001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-beta Actin antibody (ab228001) at 1/10000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 41 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded rat prostate tissue stained for beta Actin using ab228001 at 1/500 dilution in immunohistochemical analysis.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin antibody (green) using ab228001 at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold MeOH for 5 minutes.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded rat thymus gland tissue stained for beta Actin using ab228001 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat prostate tissue stained for beta Actin using ab228001 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse thymus gland tissue stained for beta Actin using ab228001 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat prostate tissue stained for beta Actin using ab228001 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse pancreas tissue stained for beta Actin using ab228001 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat thymus gland tissue stained for beta Actin using ab228001 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse pancreas tissue stained for beta Actin using ab228001 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse thymus gland tissue stained for beta Actin using ab228001 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse esophagus tissue stained for beta Actin using ab228001 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse thymus gland tissue stained for beta Actin using ab228001 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-beta Actin antibody (ab228001) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 41 kDa



    10% SDS-PAGE gel.

  • Anti-beta Actin antibody (ab228001) at 1/10000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 1 µg

    Predicted band size: 41 kDa



    10 % SDS-PAGE gel.

References

ab228001 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228001.
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