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    beta-actin-antibody-ab8227.pdf

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Isotype/Loading Controls Loading Controls Beta Actin
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Anti-beta Actin antibody (ab8227)

  • Datasheet
  • SDS
Reviews (99)Q&A (12)References (2227)

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Western blot - Anti-beta Actin antibody (ab8227)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab8227)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab8227)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab8227)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab8227)
  • Western blot - Anti-beta Actin antibody (ab8227)
  • Western blot - Anti-beta Actin antibody (ab8227)
  • Western blot - Anti-beta Actin antibody (ab8227)
  • Western blot - Anti-beta Actin antibody (ab8227)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab8227)

Key features and details

  • Rabbit polyclonal to beta Actin
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Xenopus laevis, Fish, Chinese hamster
  • Isotype: IgG

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Anti-PSAP antibody [EPR10784(B)] (ab166910)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human beta Actin protein (His tag) (ab240844)

View more associated products

Overview

  • Product name

    Anti-beta Actin antibody
    See all beta Actin primary antibodies
  • Description

    Rabbit polyclonal to beta Actin
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Xenopus laevis, Fish, Chinese hamster
    Predicted to work with: Sheep, Chicken, Guinea pig, Pig, Drosophila melanogaster, Monkey, Zebrafish, Rhesus monkey
  • Immunogen

    within Human beta Actin aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab13772)

  • Positive control

    • WB: A431, HeLa, Jurkat, HEK-293, NIH/3T3, MDCK, EBTr, SL-29, CHO and PC-12 whole cell lysate. Rat liver tissue lysate. HeLa nuclear lysate. Fish and rabbit liver. Xenopus laevis embryo. ICC: SV40LT-SMC and NIH/3T3 cells. IHC-P: Rat small intestine tissue. Human colon tissue.
  • General notes

    For western blot, milk blocking is suitable for use with fluorescent detection systems. For western blot using chemiluminescent (ECL) systems we recommend BSA blocking.

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Isotype/Loading Controls
    • Loading Controls
    • Beta Actin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Signal Transduction
    • Antibodies
    • beta actin

Associated products

  • Alternative Versions

    • Anti-Actin antibody [mAbGEa] (ab230169)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human beta Actin peptide (ab13772)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human beta Actin protein (His tag) (ab240844)
  • Related Products

    • Anti-beta Actin antibody [SP124] (ab115777)
    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-SDHB antibody [21A11AE7] (ab14714)
    • Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    • Phalloidin-iFluor 488 Reagent (ab176753)
    • Phalloidin-iFluor 647 Reagent (ab176759)
    • Anti-Actin antibody [mAbGEa] (ab230169)
    • Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
    • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    • Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

Applications

Our Abpromise guarantee covers the use of ab8227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.

W recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems.

IHC-P Use a concentration of 0.2 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We recommend Goat Anti-Rabbit IgG H&L (Biotin) (ab6720) secondary antibody or

Goat Anti-Rabbit IgG Fc (HRP) (ab97200) secondary antibody.

ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396526 Chicken
    • Entrez Gene: 280979 Cow
    • Entrez Gene: 403580 Dog
    • Entrez Gene: 60 Human
    • Entrez Gene: 11461 Mouse
    • Entrez Gene: 414396 Pig
    • Entrez Gene: 100009272 Rabbit
    • Entrez Gene: 81822 Rat
    • Entrez Gene: 574285 Rhesus monkey
    • Entrez Gene: 443052 Sheep
    • Entrez Gene: 398459 Xenopus laevis
    • Entrez Gene: 57934 Zebrafish
    • Omim: 102630 Human
    • SwissProt: P60706 Chicken
    • SwissProt: P48975 Chinese hamster
    • SwissProt: P60712 Cow
    • SwissProt: O18840 Dog
    • SwissProt: P60709 Human
    • SwissProt: P60710 Mouse
    • SwissProt: Q6QAQ1 Pig
    • SwissProt: P29751 Rabbit
    • SwissProt: P60711 Rat
    • SwissProt: O93400 Xenopus laevis
    • SwissProt: Q7ZVI7 Zebrafish
    • Unigene: 520640 Human
    • Unigene: 708120 Human
    • Unigene: 727576 Human
    • Unigene: 328431 Mouse
    • Unigene: 391967 Mouse
    • Unigene: 94978 Rat
    • Unigene: 4138 Xenopus laevis
    • Unigene: 35143 Zebrafish
    see all
  • Alternative names

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Images

  • Western blot - Anti-beta Actin antibody (ab8227)
    Western blot - Anti-beta Actin antibody (ab8227)
    All lanes : Anti-beta Actin antibody (ab8227) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) Whole Cell Lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) Whole Cell Lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab8227 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab8227)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab8227)

    ab8227 staining beta Actin in SV40LT-SMC (rat aortic smooth muscle cells transfected with SV40). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8227 at 1μg/ml (detected with ab150081, Alexa Fluor® 488 Goat anti-Rabbit, 1μg/ml, shown in green); and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab8227)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab8227)

    IHC image of ab8227 staining beta Actin in rat small intestine formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with EDTA (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab8227, 0.2μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab8227)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab8227)

    ab8227 staining beta Actin in NIH/3T3 (mouse embryo fibroblast cell line) cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8227 at 1μg/ml (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab8227)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab8227)

    IHC image of beta actin staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab8227, 1/1000 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody (ab6720, 1/1000 dilution) was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-beta Actin antibody (ab8227)
    Western blot - Anti-beta Actin antibody (ab8227)
    All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (blocked with 2% BSA)
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (blocked with 2% BSA)
    Lane 3 : Rat Liver tissue lysate (blocked with 2% BSA)
    Lane 4 : HeLa whole cell lysate (blocked with 3% Milk)
    Lane 5 : NIH/3T3 whole cell lysate (blocked with 3% Milk)
    Lane 6 : Rat Liver tissue lysate (blocked with 3% Milk)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Lanes 1-3: Blocked with 2% BSA

    Lanes 4-6: Blocked with 3% Milk

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin or 3% Milk before being incubated with ab8227 overnight at 4°C. Antibody binding was detected using an anti rabbit HRP secondary antibody, and visualised using ECL development solution ab133406

  • Western blot - Anti-beta Actin antibody (ab8227)
    Western blot - Anti-beta Actin antibody (ab8227)
    All lanes : Anti-beta Actin antibody (ab8227) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) Whole Cell Lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Performed under reducing conditions.

    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab8227 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406

  • Western blot - Anti-beta Actin antibody (ab8227)
    Western blot - Anti-beta Actin antibody (ab8227)
    All lanes : Anti-beta Actin antibody (ab8227) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 6 : HeLa nuclear lysate with Human beta Actin peptide (ab13772) at 1 µg/ml
    Lane 7 : HeLa whole cell lysate with Human beta Actin peptide (ab13772) at 1 µg/ml
    Lane 8 : A431 cell lysate with Human beta Actin peptide (ab13772) at 1 µg/ml
    Lane 9 : Jurkat cell lysate with Human beta Actin peptide (ab13772) at 1 µg/ml
    Lane 10 : HEK-293 cell lysate with Human beta Actin peptide (ab13772) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Observed band size: 41.7 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds
  • Western blot - Anti-beta Actin antibody (ab8227)
    Western blot - Anti-beta Actin antibody (ab8227)
    All lanes : Anti-beta Actin antibody (ab8227) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cells
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) cells
    Lane 3 : Fish Liver
    Lane 4 : Rabbit Liver
    Lane 5 : MDCK (Canine kidney cell line) cells
    Lane 6 : EBTr (cow trachea) cells
    Lane 7 : SL-29 (chicken day 11 embryo) cells
    Lane 8 : CHO (Chinese hamster ovary cell line) cells
    Lane 9 : Xenopus laevis embryo

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Observed band size: 41.7 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab8227)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab8227)

    IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. A anti-rabbit HRP secondary antibody (Ab97200, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (2227)

    Publishing research using ab8227? Please let us know so that we can cite the reference in this datasheet.

    ab8227 has been referenced in 2227 publications.

    • Shi H  et al. Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression. Oncogene 39:530-545 (2020). PubMed: 31501521
    • Yang SY  et al. Glucocerebrosidase activity, cathepsin D and monomeric a-synuclein interactions in a stem cell derived neuronal model of a PD associated GBA1 mutation. Neurobiol Dis 134:104620 (2020). PubMed: 31634558
    • Koeberle SC  et al. Distinct and overlapping functions of glutathione peroxidases 1 and 2 in limiting NF-?B-driven inflammation through redox-active mechanisms. Redox Biol 28:101388 (2020). PubMed: 31765890
    • Mao P  et al. PDZ-Binding Kinase-Dependent Transcriptional Regulation of CCNB2 Promotes Tumorigenesis and Radio-Resistance in Glioblastoma. Transl Oncol 13:287-294 (2020). PubMed: 31874375
    • Xie S  et al. Andrographolide Protects Against Adverse Cardiac Remodeling After Myocardial Infarction through Enhancing Nrf2 Signaling Pathway. Int J Biol Sci 16:12-26 (2020). PubMed: 31892842
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 111 Abreviews or Q&A

    Western blot abreview for Anti-beta Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Zebrafish Tissue lysate - whole (Larvae 5hpf)
    Specification
    Larvae 5hpf
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Mr. Pedro Guedes Dias

    Verified customer

    Submitted Aug 22 2014

    Western blot abreview for Anti-beta Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Rat Cell lysate - whole cell (Primary Neurons)
    Specification
    Primary Neurons
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 22 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-beta Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Mouse Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    Yes - 0,3% TritonX
    Fixative
    Perfusion fixed with paraformaldehyde and picric acid
    Read More

    Mr. Frank Rommel

    Verified customer

    Submitted Aug 08 2014

    Western blot abreview for Anti-beta Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (Biorad Criterion TGX Stain Free Gel, Any kD, Cat. number 5678123)
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Drosophila heads)
    Specification
    Drosophila heads
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 02 2014

    Western blot abreview for Anti-beta Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Rat Tissue lysate - whole (ovaries)
    Specification
    ovaries
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Read More

    Mrs. Henda Nabli

    Verified customer

    Submitted Oct 01 2013

    Question

    Does this b-actin antibody stain nuclear pools of actin? The demonstrative pictures show great cytoplasmic staining but it is unclear if there is nuclear staining.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 20 2012

    Answer

    We do not know if ab8227 stains nuclear actin. It is raised against a sequence from cytoplasmic actin, and we have not tested for cross-reactivity with nuclear actin.

    http://www.uniprot.org/uniprot/P60709

    Read More

    Abcam Scientific Support

    Answered on Sep 20 2012

    Question

    Would ab8227 work with either ab79667 or ab113279 for a sELISA?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 18 2012

    Answer

    I just received an update form lab regarding the epitope for ab79667 and ab113279. Unfortunately the epitope has not been determined so the information is not available. I am sorry we will not be able to suggest these to be paired with ab8227 in sELISA.

    Read More

    Abcam Scientific Support

    Answered on Jul 18 2012

    Question

    We’d like to determine if the issues we are having with ab8227 are caused by a problem with the biology or a problem with your antibody itself. Can you tell me what you used as quality control for this antibody?  

    Read More

    Abcam community

    Verified customer

    Asked on Jan 25 2012

    Answer

    I have had the opportunity to discuss our QC methods regarding this product with the lab. Ab17721 is batch tested by us in WB in HeLa, Jurkat and NIH3T3 lysates and also in Immunofluorescence in HeLa cells. Each new AP purified must pass both of these applications to be put into stock.

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    Abcam Scientific Support

    Answered on Jan 25 2012

    Western blot abreview for Anti-beta Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (iPSC-derived microglia)
    Gel Running Conditions
    Reduced Denaturing (10% Tris Bolt)
    Loading amount
    30 µg
    Specification
    iPSC-derived microglia
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Boyd Kenkhuis

    Verified customer

    Submitted May 06 2020

    Western blot abreview for Anti-beta Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (PC12 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    PC12 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
    Read More

    Dr. Alexandre Magno

    Verified customer

    Submitted Jun 27 2019

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