Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Zebrafish Tissue lysate - whole (Larvae 5hpf)
Specification
Larvae 5hpf
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: BSA 5% PBS-T 0.05%
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL+
Bands
Specific: 45 47 kDa Non-specific: 38 kDa
Exposure
8 minute(s) and 20 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Pedro Guedes Dias
Verified customer
Submitted Aug 22 2014