Application
Western blot
Sample
Human Tissue lysate - whole (Grey matter from BA20 (temporal cortex))
Loading amount
5 µg
Specification
Grey matter from BA20 (temporal cortex)
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/50000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% NFDM in PBST
Secondary antibody
Name
Non-Abcam antibody was used: Alexoflur 680 goat anti-mouse
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 680
Dilution
1/5000
Detection
Detection method
Odyssey (infrared) intensity 1
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 42 kDa
Additional data
Additional Notes
Actin ab used as a loading control. ab gives single clean band at predicted weight. Bands below actin in image are synaptophysin (SYN).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Mark Elliott
Verified customer
Submitted Apr 22 2008