Application
Western blot
Sample
Human Cell lysate - whole cell (T-cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris HCl)
Loading amount
10.6 µg
Specification
T-cells
Blocking step
Licor intercept TBS as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Licor intercept TBS
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 800CW Donkey anti-mouse (Li-Cor REF: 925-322
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 790
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 790
Detection
Detection method
fluorescence
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 40 kDa Non-specific: 50 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Feb 20 2020